Eco-friendly biosynthesis of TiO2 nanoparticles using Desmostachya bipinnata extract: Larvicidal and pupicidal potential against Aedes aegypti and Spodoptera litura and acute toxicity in non-target organisms

Abstract

The resistance to insecticides among insects, including mosquitoes and agricultural pests, and the impact of these compounds' environmental risks and health issues have motivated the proposition of eco-friendly alternatives. Thus, we aimed to explore the potential use of Desmostachya bipinnata for the biosynthesis of TiO₂NPs and evaluate their larvicidal and pupicidal activity of target (Aedes aegypti and Spodoptera litura) and acute toxicity in non-target organisms (Toxorhynchites splendens and Eisenia fetida), at distinct concentrations, after 24 h of exposure. The characterization of the biosynthesized TiO₂NPs was carried out by FT-IR, XRD, SEM, and EDX analysis. Under the UV-vis spectrum analysis, a sharp peak was recorded at 200 to 800 nm, which indicated the production of TiO₂NPs by the plant extract. The SEM analysis revealed that the synthesized TiO₂NPs were spherical with a diameter of 36.4 nm and were detected in the XRD spectrum analysis related to the TiO₂NPs. The highest percentage of mortality recorded at 900 μg/mL was 96 % and 94 % in the 2nd instar of A. aegypti and S. litura larvae, respectively, and exhibited the LC₅₀ and LC₉₀ values 5 of 458.79 and 531.01 μg/mL, respectively. The biosynthesized TiO₂NPs showed concentration-dependent increased pupal lethality for both A. aegypti and S. litura. We also observed increased detoxification enzyme activity (α esterase, β esterase, and glutathione-S-transferase) of A. aegypti and S. litura exposed to different concentrations of biosynthesized TiO₂NPs as histopathological changes in the midgut region of these animals. On the other hand, the mortality rate of non-target organisms (T. splendens and E. fetida) was lower when exposed to TiO₂NPs, compared to the high lethality induced by synthetic pesticides (cypermethrin and monocrotophos for E. fetida; and cypermethrin and temphos for T. splendens). Thus, our study provides pioneering evidence on the potential use of D. bipinnata-mediated TiO₂NPs for controlling mosquito vectors and agricultural pest management.

Keywords: Agriculture pest; Detoxification enzyme; Earthworm; Mosquito vector; Plant extract; TiO(2)NPs; Toxicity.