Simulated digestion, dynamic changes during fecal fermentation and effects on gut microbiota of Avicennia marina (Forssk.) Vierh. fruit non-starch polysaccharides

Qingxia Yuan; Kunling Lv; Jinwen Huang; Shujing Sun; Ziyu Fang; Hongjie Tan; Hong Li; Dan Chen; Longyan Zhao; Chenghai Gao; Yonghong Liu

doi:10.1016/j.fochx.2022.100475

Simulated digestion, dynamic changes during fecal fermentation and effects on gut microbiota of Avicennia marina (Forssk.) Vierh. fruit non-starch polysaccharides

Food Chem X. 2022 Oct 13:16:100475. doi: 10.1016/j.fochx.2022.100475. eCollection 2022 Dec 30.

Authors

Qingxia Yuan¹, Kunling Lv^{1

2}, Jinwen Huang¹, Shujing Sun¹, Ziyu Fang¹, Hongjie Tan¹, Hong Li¹, Dan Chen³, Longyan Zhao¹, Chenghai Gao¹, Yonghong Liu¹

Affiliations

¹ Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China.
² College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, Guangxi, China.
³ College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.

Abstract

Grey mangrove (Avicennia marina (Forssk.) Vierh.) fruit is a traditional folk medicine and health food consumed in many countries. In this study, its polysaccharides (AMFPs) were obtained and analyzed by chemical and instrumental methods, with the results indicating that AMFPs consisted of galactose, galacturonic acid, arabinose, and rhamnose in a molar ratio of 4.99:3.15:5.38:1.15. The dynamic changes in AMFPs during the digestion and fecal fermentation processes were then investigated. The results confirmed that AMFPs were not depolymerized by gastric acid and various digestive enzymes. During fermentation, 56.05 % of the AMFPs were utilized by gut microbiota. Galacturonic acid, galactose, and arabinose from AMFPs, were mostly consumed by gut microbiota. AMFPs obviously decreased harmful bacteria and increased some beneficial microbiota, including Megasphaera, Mistuokella, Prevotella, and Megamonas. Furthermore, AMFPs obviously increased the levels of various short-chain fatty acids. These findings suggest that AMFPs have potential prebiotic applications for improving gut health.

Keywords: 3,5-Dinitrosalicylic acid (PubChem CID: 5251731); 3-Methyl-1-phenyl-2-pyrazolin-5-one (PubChem CID: 4021); Acetic acid (PubChem CID: 176); Avicennia marina (Forssk.) Vierh. fruit polysaccharides; Deuterium oxide (PubChem CID: 24602); Digestion; Fermentation; Fructooligosaccharide (PubChem CID: 439709); Grey mangrove; Gut microbiota; Propionic acid (PubChem CID: 1032); d-Galactose (PubChem CID: 6036); d-Galacturonic Acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Rhamnose (PubChem CID: 5460029).