Conjugation of cysteamine functionalized nanodiamond to gold nanoparticles for pH enhanced colorimetric detection of Cr3+ ions demonstrated by real water sample analysis

Abstract

A cysteamine functionalized nanodiamond (NDC) was conjugated to gold nanoparticles (AuNPs) to deliver NDC@AuNPs and utilized in enhanced colorimetric detection of Cr³⁺ at pH 6 environment. The conjugation was validated using FTIR, TEM, PXRD, DLS, and zeta potential investigations. At pH 6, superior sensory response of NDC@AuNPs to Cr³⁺ than that of other ions was validated by UV-vis spectroscopy and colorimetric photographs. Results from UV-vis titrations displayed a linear regression from 0.01 to 0.4 µM with a LOD of 0.236 ± 0.005 nM. The particle aggregation, size variations, potential changes, and binding modes are investigated using TEM, DLS, and FTIR techniques to explore the underlying mechanisms. By adding the EDTA, sensory response is reversible up to 4 cycles. Finally, spiked real water experiments show improved sensing of Cr³⁺ at pH 6 via the observed recovery between 96 and 110 %, which is in good agreement with the ICP-mass data.

Keywords: Conjugated AuNPs; Cr ion; Cysteamine capped nanodiamond; Nanomolar detection; Real water studies; Reversibility; pH enhanced quantitation.