Development of new tools to study membrane-anchored mammalian Atg8 proteins

Sang-Won Park; Pureum Jeon; Akinori Yamasaki; Hye Eun Lee; Haneul Choi; Ji Young Mun; Yong-Woo Jun; Ju-Hui Park; Seung-Hwan Lee; Soo-Kyeong Lee; You-Kyung Lee; Hyun Kyu Song; Michael Lazarou; Dong-Hyong Cho; Masaaki Komatsu; Nobuo N Noda; Deok-Jin Jang; Jin-A Lee

doi:10.1080/15548627.2022.2132040

Development of new tools to study membrane-anchored mammalian Atg8 proteins

Autophagy. 2023 May;19(5):1424-1443. doi: 10.1080/15548627.2022.2132040. Epub 2022 Oct 17.

Authors

Sang-Won Park¹, Pureum Jeon², Akinori Yamasaki³, Hye Eun Lee⁴, Haneul Choi², Ji Young Mun⁴, Yong-Woo Jun⁵, Ju-Hui Park⁵, Seung-Hwan Lee⁵, Soo-Kyeong Lee², You-Kyung Lee², Hyun Kyu Song⁶, Michael Lazarou⁷, Dong-Hyong Cho⁸, Masaaki Komatsu⁹, Nobuo N Noda^{3

10}, Deok-Jin Jang^{1

5}, Jin-A Lee²

Affiliations

¹ Department of Vector Entomology, College of Ecology and Environment, Kyungpook National University, Sangju, Korea.
² Department of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University, Daejeon, Korea.
³ Institute of Microbial Chemistry, Tokyo, Japan.
⁴ Neural circuit research group, Korea Brain Research Institute, Daegu, Korea.
⁵ Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju, Korea.
⁶ Department of Life Sciences, Korea University, Seoul, Korea.
⁷ Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Victoria, Australia.
⁸ School of Life Sciences, Kyungpook National University, Daegu, Korea.
⁹ Department of Physiology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
¹⁰ Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.

Abstract

A:C autophagic membrane:cytosol; ALS amyotrophic lateral sclerosis; ATG4 autophagy related 4; Atg8 autophagy related 8; BafA1 bafilomycin A₁; BNIP3L/Nix BCL2 interacting protein 3 like; CALCOCO2/NDP52 calcium binding and coiled-coil domain 2; EBSS Earle's balanced salt solution; GABARAP GABA type A receptor-associated protein; GST glutathione S transferase; HKO hexa knockout; K_d dissociation constant; LIR LC3-interacting region; MAP1LC3/LC3 microtubule associated protein 1 light chain 3; NLS nuclear localization signal/sequence; PE phosphatidylethanolamine; SpHfl1 Schizosaccharomyces pombeorganic solute transmembrane transporter; SQSTM1/p62 SQSTM1/p62; TARDBP/TDP-43 TAR DNA binding protein; TKO triple knockout.

Keywords: Autophagy; GABARAP; LIR motif; RavZ protein; mammalian ATG8; selective mATG8–PE delipidation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis Regulatory Proteins / metabolism
Autophagy* / genetics
Autophagy-Related Protein 8 Family / metabolism
Mammals / metabolism
Membrane Proteins* / metabolism
Microtubule-Associated Proteins / metabolism
Sequestosome-1 Protein / metabolism

Substances

Autophagy-Related Protein 8 Family
Membrane Proteins
Sequestosome-1 Protein
Apoptosis Regulatory Proteins
Microtubule-Associated Proteins

Grants and funding

The work was supported by the Neurological Disorder Research Program of the National Research Foundation (NRF) [NRF-2020M3E5D9079908]; Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT [2017M3A9G7073521]; Basic science research program through the NRF, funded by the Ministry of Education [2021R1A6A3A13039924]; KBRI basic research program through Korea Brain Research Institute funded by the Ministry of Science and ICT [22-BR-01-03]; Grant-in-Aid for Scientific Research on Innovative Areas [19H05706]; Grant-in-Aid for Scientific Research (B) [18H02611]; Japan Society for the Promotion of Science Takeda Science Foundation Advanced Research and Development Programs for Medical Innovation [AMED-CREST, 22gm1410004s0103]; JSPS KAKENHI Grant-in-Aid for Scientific Research [18H03989 and 19H05707]; JST CREST [JPMJCR20E3]; Japan Agency for Medical Research and Development [BINDS grant JP19am0101001, support number 0002]; Basic science research program of the NRF [NRF-2018R1D1A1B07048822]; Basic Research Program of the NRF [NRF-2020R1A2C2005021]; Science Research Center Program of the NRF [2020R1A5A1019023]; Basic science research program through the NRF, funded by the Ministry of Education [2022R1A6A3A13071799]