The combinatory effect of scaffold topography and culture condition: an approach to nucleus pulposus tissue engineering

Noviana Vanawati; Anggraini Barlian; Hermawan Judawisastra; Indra Wibowo

doi:10.2144/fsoa-2021-0157

The combinatory effect of scaffold topography and culture condition: an approach to nucleus pulposus tissue engineering

Future Sci OA. 2022 Oct 3;8(7):FSO810. doi: 10.2144/fsoa-2021-0157. eCollection 2022 Aug.

Authors

Noviana Vanawati¹, Anggraini Barlian¹, Hermawan Judawisastra², Indra Wibowo¹

Affiliations

¹ School of Life Sciences & Technology, Institut Teknologi Bandung, Bandung, West Java, 40132, Indonesia.
² Faculty of Mechanical & Aerospace Engineering, Institut Teknologi Bandung, Bandung, West Java, 40132, Indonesia.

Abstract

Scaffold topography and culture medium conditions for human wharton's jelly mesenchymal stem cells (hWJ-MSC) are critical components of the approach to nucleus pulposus (NP) tissue engineering.

Aim: To evaluate the silk fibroin (SF) scaffold topography analysis (optimal thickness and pore diameter) and to determine culture medium conditions for the growth and differentiation of hWJ-MSC.

Method: hWJ-MSCs were seeded into different thicknesses and pore size diameters and grown in different concentrations of glucose, platelet rich plasma (PRP) and oxygen. The cell-seeded scaffold was evaluated for cell attachment, growth and differentiation potency.

Results & discussion: The results indicated that SF scaffold with a minimum thickness 3.5 mm and pore diameter of 500 μm with cells cultured under low glucose, 10% PRP and normoxia conditions induced the growth and differentiation of hWJ-MSCs, indicated by the accumulation of glycosaminoglycans content and the presence of type II collagen, as markers of NP-like cells.

Keywords: Human Wharton's jelly mesenchymal stem cells; hypoxia; low oxygen concentration; microenvironment; nucleus pulposus tissue engineering; platelet rich plasma; silk fibroin scaffold.