DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore

Rong Tian; Ting Weng; Shanchuan Chen; Ji Wu; Bohua Yin; Wenhao Ma; Liyuan Liang; Wanyi Xie; Yunjiao Wang; Xiaoqing Zeng; Yajie Yin; Deqiang Wang

doi:10.1016/j.bioelechem.2022.108284

DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore

Bioelectrochemistry. 2023 Feb:149:108284. doi: 10.1016/j.bioelechem.2022.108284. Epub 2022 Oct 8.

Authors

Rong Tian¹, Ting Weng¹, Shanchuan Chen¹, Ji Wu¹, Bohua Yin², Wenhao Ma¹, Liyuan Liang¹, Wanyi Xie¹, Yunjiao Wang¹, Xiaoqing Zeng³, Yajie Yin⁴, Deqiang Wang⁵

Affiliations

¹ Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
² Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China; Changchun University of Science and Technology, Changchun, China.
³ Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China; Chongqing University, Chongqing, China.
⁴ Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China. Electronic address: yinyajie@cigit.ac.cn.
⁵ Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China. Electronic address: dqwang@cigit.ac.cn.

PMID: 36244111
DOI: 10.1016/j.bioelechem.2022.108284

Abstract

In this paper, a novel detection technique for tumor marker carcinoembryonic antigen (CEA) has been developed by using a solid-state nanopore as a tool. The system utilizes the specific affinity between aptamer-modified magnetic Fe₃O₄ and CEA, rather than directly detecting the translocation of CEA through the nanopore. The aptamer-modified magnetic Fe₃O₄ was hybridized with tetrahedral DNA nanostructures (TDNs), and TDNs were released after CEA was added. We investigate the translocation behavior of individual TDNs through solid-state nanopores. The frequency of the blockage signals for TDNs is recorded for indirect detection of CEA. We realized the detection of CEA with a concentration as low as 0.1 nM and proved the specificity of the interaction between the aptamer. In addition, our designed nanopore sensing strategy can detect CEA in real samples.

Keywords: Aptamer; Carcinoembryonic antigen; Solid-state nanopore; Tetrahedral DNA.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Carcinoembryonic Antigen
DNA / chemistry
Nanopores*
Nanostructures

Substances

Aptamers, Nucleotide
Carcinoembryonic Antigen
DNA