Osmoprotectants boost adaptation and protect methanogenic microbiome during ammonia toxicity events in continuous processes

Yixin Yan; Miao Yan; Irini Angelidaki; Dafang Fu; Ioannis A Fotidis

doi:10.1016/j.biortech.2022.128106

Osmoprotectants boost adaptation and protect methanogenic microbiome during ammonia toxicity events in continuous processes

Bioresour Technol. 2022 Nov:364:128106. doi: 10.1016/j.biortech.2022.128106. Epub 2022 Oct 13.

Authors

Yixin Yan¹, Miao Yan², Irini Angelidaki³, Dafang Fu⁴, Ioannis A Fotidis⁵

Affiliations

¹ School of Civil Engineering, Southeast University, 210096 Nanjing, China; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
² Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
³ Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
⁴ School of Civil Engineering, Southeast University, 210096 Nanjing, China.
⁵ School of Civil Engineering, Southeast University, 210096 Nanjing, China; Faculty of Environment, Ionian University, 29100 Zakynthos, Greece. Electronic address: ifotidis@ionio.gr.

PMID: 36243262
DOI: 10.1016/j.biortech.2022.128106

Abstract

Different osmoprotectants were used to counteract ammonia toxicity in continuous anaerobic reactors. The anaerobic microbiome osmoadaptation process and its role to the methanogenic recovery are also assessed. Three osmoprotectants (i.e., glycine betaine, MgCl₂ and KCl) were respectively introduced in continuous reactors at high ammonia levels, namely R_GB, R_Mg, R_K, while a control reactor (R_Ctrl) was also used. After ammonia was introduced, the R_GB, R_Mg, R_K and R_Ctrl suffered 39.0%, 36.6%, 39.9% and 36.2% methane production loss, respectively. Osmoprotectants addition recovered significantly methane production by up to 68.9%, 54.3% and 32.2% for R_GB, R_Mg and R_K, respectively contrary to R_Ctrl, where production increased only by 13.6%. The recovered methane production was maintained in R_GB and R_Mg for at least four HRTs, even after the addition of osmoprotectants was stopped, due to the formed methanogenic microbiota by osmoadaptation process, with Methanoculleus sp. as the dominant species.

Keywords: Ammonia inhibition; Glycine betaine; Magnesium chloride; Methanoculleus thermophilus; Osmoadaptation.

MeSH terms

Ammonia
Anaerobiosis
Bioreactors
Euryarchaeota*
Methane
Microbiota*

Substances

Ammonia
Methane