A hormesis-like effect of FeS on heterotrophic denitrification and its mechanisms

Abstract

To alleviate the insufficiency of carbon source in sewage, many sulfur-containing inorganic electron donors were added into traditional heterotrophic denitrification process. However, the effects of extraneous inorganic electron donors on heterotrophic denitrification were still largely unknown. In this study, a hormesis-like effect of ferrous sulfide (FeS, a representative inorganic electron donors) on Paracoccus denitrificans was observed. Total nitrogen (TN) removal efficiency of P. denitrificans rose by 15% with the increase of FeS dosage from 0 to 0.3 g L^-1 (low level), whereas the TN removal significantly decreased to 53% as the dosage of FeS mounted up to 5.0 g L^-1 (high level). Furthermore, the impacts of FeS on glucose utilization and bacterial growth exhibited hormesis-like effects. A subsequent mechanistic study revealed that above influences were caused by its released ions (Fe²⁺, Fe³⁺, and S^2-) rather than particle size. Further study illustrated that low dosage of FeS released a small amount of Fe²⁺ and Fe³⁺, which provided sufficient electrons via promoting glucose utilization, then improved denitrification. Conversely, FeS with high dosage inhibited denitrification via its released S^2-, which suppressed the activity of key denitrifying enzymes rather than influenced glucose metabolism and electron provision. Our results provide an insight into improving denitrification efficiency of the mixotrophic process coexisting with autotrophic and heterotrophic denitrifiers.

Keywords: Autotrophic denitrification; Carbon source; Electron donor; FeS; Heterotrophic denitrification.