Structure and antiproliferative activity of the polysaccharide from Halomonas aquamarina related to Cobetia pacifica

Maxim S Kokoulin; Elena N Sigida; Alexandra S Kuzmich; Ibrahim M Ibrahim; Yulia P Fedonenko; Svetlana A Konnova

doi:10.1016/j.carbpol.2022.120125

Structure and antiproliferative activity of the polysaccharide from Halomonas aquamarina related to Cobetia pacifica

Carbohydr Polym. 2022 Dec 15:298:120125. doi: 10.1016/j.carbpol.2022.120125. Epub 2022 Sep 19.

Authors

Maxim S Kokoulin¹, Elena N Sigida², Alexandra S Kuzmich¹, Ibrahim M Ibrahim³, Yulia P Fedonenko⁴, Svetlana A Konnova⁴

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospekt 100 let Vladivostoku, Vladivostok 690022, Russia.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, FRC Saratov Scientific Centre of Russian Academy of Sciences, 13, Prospekt Entuziastov, Saratov 410049, Russia. Electronic address: si_elena@mail.ru.
³ Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt; Chernyshevky Saratov State University, 83, Ulitsa Astrakhanskaya, Saratov 410012, Russia.
⁴ Institute of Biochemistry and Physiology of Plants and Microorganisms, FRC Saratov Scientific Centre of Russian Academy of Sciences, 13, Prospekt Entuziastov, Saratov 410049, Russia; Chernyshevky Saratov State University, 83, Ulitsa Astrakhanskaya, Saratov 410012, Russia.

PMID: 36241297
DOI: 10.1016/j.carbpol.2022.120125

Abstract

Here, the results of the structure and the activity of capsular polysaccharides isolated from the Halomonas aquamarina EG27S8QL and Cobetia pacifica KMM3878 have been described. Both polysaccharides were studied by spectroscopic and chemical methods and were found to be structurally related sulfated galactans differing in the position of the sulfate group: →6)-β-D-Galp3S-(1 → 4)-β-D-Galp3S-(1 → 6)-β-D-Galp3,4(S-Pyr)-(1 → [H. aquamarina EG27S8QL] →6)-β-D-Gal-(1 → 4)-β-D-Gal2,3S-(1 → 6)-β-D-Gal3,4(S-Pyr)-(1 → [C. pacifica KMM3878] Structure of the CPS from H. aquamarina EG27S8QL has not been hitherto reported, whereas the CPS from C. pacifica KMM3878 was identical to the previously studied O-polysaccharide. The CPSs exhibited an antiproliferative effect and suppressed the colony formation of DLD-1 and MCF-7 cells in a different manner.

Keywords: Antiproliferative activity; Capsular polysaccharide structure; Cobetia; Halomonas; Halotolerant bacterium; Sulfate.

MeSH terms

Galactans
Halomonadaceae
Halomonas
Humans
Polysaccharides* / pharmacology
Sulfates* / chemistry

Substances

Galactans
Polysaccharides
Sulfates

Supplementary concepts

Cobetia pacifica
Halomonas aquamarina