Characterization of the complete chloroplast genome of Clematis orientalis (Ranunculaceae)

Yi Cui; Lihua Yang; Bingru Ma; Shaoping Ling; Jingwen Wang; Zhongming Han; Yunhe Wang

doi:10.1080/23802359.2022.2127339

Characterization of the complete chloroplast genome of Clematis orientalis (Ranunculaceae)

Mitochondrial DNA B Resour. 2022 Oct 6;7(10):1773-1775. doi: 10.1080/23802359.2022.2127339. eCollection 2022.

Authors

Yi Cui^{1

2}, Lihua Yang³, Bingru Ma^{1

2}, Shaoping Ling^{1

2}, Jingwen Wang^{1

2}, Zhongming Han^{1

2}, Yunhe Wang^{1

2}

Affiliations

¹ College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, PR China.
² State Key Laboratory of JLP-MOST for Ecological Restoration and Ecosystem Management, Changchun, PR China.
³ College of Life Science, Changchun Sci-Tech University, Changchun, PR China.

Abstract

The medicinal plant Clematis orientalis L. belongs to the family Ranunculaceae. In this study, we determined the complete chloroplast genome sequence of C. orientalis and its phylogenetic relationships with other species. The complete chloroplast genome of C. glauca is 159,518 bp in length, circular in structure, and has four regions including a large single-copy (LSC) region of 79,453 bp; a small single-copy (SSC) region of 17,997 bp; and two inverted repeat (IR) regions of 31,034 bp. The GC content of the genome is 38%, and those of LSC, SSC, and IR regions are 36.2, 31.4, and 42%, respectively. The genome encodes 129 unique genes, including 85 protein-coding genes, 36 tRNA genes, and eight rRNA genes. Phylogenomic analysis reveals that C. orientalis is most closely related to C. aethusifolia. This study contributes to better understanding of phylogenetic relationships of Ranunculaceae.

Keywords: Clematis orientalis; Ranunculaceae; chloroplast genome; phylogenetic tree.

Grants and funding

This project is funded by the National Key R&D Program of China [2019YFC1710700, 2019YFC1710702], Scientific and Technological Planning Project of Jilin Province [20200404010YY, 20200708033YY], and China Agriculture Research System of MOF and MARA [CARS-21].