Tristaenone A: A New Anti-Inflammatory Compound Isolated from the Australian Indigenous Plant Tristaniopsis laurina

Shintu Mathew; Xian Zhou; Gerald Münch; Francis Bodkin; Matthew Wallis; Feng Li; Ritesh Raju

doi:10.3390/molecules27196592

Tristaenone A: A New Anti-Inflammatory Compound Isolated from the Australian Indigenous Plant Tristaniopsis laurina

Molecules. 2022 Oct 5;27(19):6592. doi: 10.3390/molecules27196592.

Authors

Shintu Mathew^{1

2}, Xian Zhou¹, Gerald Münch^{1

2}, Francis Bodkin², Matthew Wallis³, Feng Li³, Ritesh Raju²

Affiliations

¹ NICM Health Research Institute, Western Sydney University, Penrith, NSW 2751, Australia.
² Department of Pharmacology, Western Sydney University, Campbelltown Campus, Sydney, NSW 2560, Australia.
³ School of Science, Western Sydney University, Penrith, NSW 2751, Australia.

Abstract

Inspired by ethnopharmacological knowledge, we conducted a bioassay-guided fractionation of the leaves of Tristaniopsis laurina which led to the discovery of a new anti-inflammatory compound, tristaenone A (1). The structure was elucidated by detailed spectroscopic data analysis, and the absolute configuration was established using X-ray crystallography analysis. Tristaenone A (1) suppressed LPS and IFN-γ-induced NO, TNF-α and IL-6 production in RAW 264.7 cells with IC₅₀ values of 37.58 ± 2.45 μM, 80.6 ± 5.82 μM and 125.65 ± 0.34 μM, respectively. It also inhibited NF-κB nuclear translocation by 52.93 ± 14.14% at a concentration of 31.85 μM.

Keywords: Australian indigenous plant; anti-inflammatory; flavonoids; hydroxycyclohexenone; nitric oxide (NO); nuclear factor kappa B (NF-κB).

MeSH terms

Animals
Anti-Inflammatory Agents / pharmacology
Australia
Interleukin-6 / metabolism
Lipopolysaccharides* / pharmacology
Mice
NF-kappa B* / metabolism
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / metabolism
RAW 264.7 Cells
Tumor Necrosis Factor-alpha / metabolism

Substances

Anti-Inflammatory Agents
Interleukin-6
Lipopolysaccharides
NF-kappa B
Tumor Necrosis Factor-alpha
Nitric Oxide
Nitric Oxide Synthase Type II

Grants and funding

This research received no external funding.