RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway

Jie Xu; Pengfei Shi; Fanwei Xia; Xuan Zhao; Junfan Chen; Rui Geng; Hongjuan Cui; Liqun Yang

doi:10.3390/ijms231912036

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway

Int J Mol Sci. 2022 Oct 10;23(19):12036. doi: 10.3390/ijms231912036.

Authors

Jie Xu^{1

2}, Pengfei Shi^{1

2}, Fanwei Xia^{1

2}, Xuan Zhao^{1

2}, Junfan Chen^{1

2}, Rui Geng^{1

2}, Hongjuan Cui^{1

2}, Liqun Yang^{1

2}

Affiliations

¹ State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
² Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China.

Abstract

Melanoma originates from the malignant transformation of melanocytes. Compared with other skin cancers, melanoma has a higher fatality rate. The 5-year survival rate of patients with early-stage primary melanoma through surgical resection can reach more than 90%. However, the 5-year survival rate of patients with metastatic melanoma is only 25%. Therefore, accurate assessment of melanoma progression is critical. Previous studies have found that Retinoic Acid Induced 14(RAI14) is critical in tumorigenesis. However, the biological function of RAI14 for the development of melanoma is unclear. In this study, RAI14 is highly expressed in melanoma and correlated with prognosis. The expression of RAI14 can affect the proliferation, migration and invasion of melanoma cells. F-Box Protein 32(FBXO32) is an E3 ubiquitin ligase of c-MYC. We found that RAI14 affects the transcriptional expression of FBXO32 and regulates the stability of c-MYC. These results suggest that RAI14 play an important role in the growth of melanoma and is expected to be a therapeutic target for melanoma.

Keywords: RAI14; c-MYC; melanoma; proliferation; ubiquitination.

MeSH terms

Cell Proliferation / genetics
Cell Transformation, Neoplastic
Cytoskeletal Proteins* / metabolism
F-Box Proteins* / genetics
F-Box Proteins* / metabolism
Humans
Melanoma* / genetics
Melanoma* / pathology
Muscle Proteins / metabolism
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism
SKP Cullin F-Box Protein Ligases / metabolism
Skin Neoplasms*
Transcription Factors* / metabolism
Tretinoin / metabolism
Ubiquitin-Protein Ligases / metabolism

Substances

Cytoskeletal Proteins
F-Box Proteins
MYC protein, human
Muscle Proteins
Proto-Oncogene Proteins c-myc
RAI14 protein, human
Transcription Factors
Tretinoin
FBXO32 protein, human
SKP Cullin F-Box Protein Ligases
Ubiquitin-Protein Ligases

Grants and funding

This research was supported by the Natural Science Foundation of Chongqing (cstc2022ycjh-bgzxm0145).