The Laotian shad (Tenualosa thibaudeaui) belongs to the family Clupeidae and is mainly distributed across Lao PRD, Cambodia, and northern Thailand. Due to overfishing and dam reconstruction, the Laotian shad is on the verge of extinction and currently listed as vulnerable by the IUCN. Nanopore and Illumina sequencing data were integrated to generate the first high-quality genome assembly for T. thibaudeaui. The assembled genome was 638 Mb in size, including 228 scaffolds with a N50 value of 16.6 Mb. BUSCO analysis revealed the completeness of the assembly to be more than 96%. A total of 24,810 protein-coding genes were predicted. According to the pairwise sequentially Markovian coalescent analysis, the effective population size of the Laotian shad sharply declined from 3 Mya to 20 Kya. We found a significant ratio in contraction of gene families that may reflect secondary gene loss. Our high-quality genome assembly of the Laotian shad will provide a valuable resource for future research in conservation genetics, as well as for investigating the phylogenetics and comparative genomics of shads.
Keywords: Laotian shad; Nanopore sequencing; effective population size; gene families.
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