Mechanistic insights into aggregation process of graphene oxide and bacterial cells in microbial reduction of ferrihydrite

Abstract

Microbial reduction of ferrihydrite is prevalent in natural environments and plays an important role in reductive dissolution of Fe(III) minerals. With consistent release of anthropogenic graphene oxide (GO) into water bodies, new changes in the Fe(III)-reducing microorganisms/ferrihydrite binary system demand attention. Herein, we focused on the interaction of GO and bacterial cells in view of colloidal stability and interfacial forces, and on the consequences for microbial ferrihydrite reduction. The results showed that the addition of GO decreased the bioreduction efficiency of ferrihydrite down to 1/15 of the control. Meanwhile, the GO nanosheets were found not depositing on ferrihydrite but spontaneously aggregating with Shewanella spp., the representative dissimilatory Fe(III) reduction bacterial species. Using the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory and atomic force microscopy (AFM), the aggregation process can be interpreted in three steps according to the interaction energy calculation, namely, colloidal instability, reversible aggregation and irreversible aggregation. The motility of living cells seems the reason inducing the colloidal instability between GO and bacteria. While, the aggregation remains reversible even the secondary minimum achieved at the separation distance of 8.74-9.24 nm from XDLVO. When the separation distance <5.74-6.01 nm, the adhesion work predominates and causes irreversible aggregation, validated by AFM. Additionally, the probable ecological risks raised by this aggregation behavior for the imbalance of iron biogeochemical cycle were demonstrated.

Keywords: AFM; Aggregation; Graphene oxide; Microbial reduction of ferrihydrite; Shewanella; XDLVO.