Mycosynthesis of highly fluorescent selenium nanoparticles from Fusarium oxysporum, their antifungal activity against black fungus Aspergillus niger, and in-vivo biodistribution studies

Sk Najrul Islam; Syed Mohd Adnan Naqvi; Azam Raza; Amit Jaiswal; Akhilesh K Singh; Manish Dixit; Atul Barnwal; Sanjay Gambhir; Absar Ahmad

doi:10.1007/s13205-022-03383-0

Mycosynthesis of highly fluorescent selenium nanoparticles from Fusarium oxysporum, their antifungal activity against black fungus Aspergillus niger, and in-vivo biodistribution studies

3 Biotech. 2022 Nov;12(11):309. doi: 10.1007/s13205-022-03383-0. Epub 2022 Oct 5.

Authors

Sk Najrul Islam¹, Syed Mohd Adnan Naqvi¹, Azam Raza¹, Amit Jaiswal², Akhilesh K Singh², Manish Dixit², Atul Barnwal², Sanjay Gambhir², Absar Ahmad¹

Affiliations

¹ Interdisciplinary Nanotechnology Centre (INC), Z. H. College of Engineering and Technology, Aligarh Muslim University, AMU, Aligarh, UP 202002 India.
² Department of Nuclear Medicine, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow, UP 22014 India.

Abstract

In the past few years, photo-luminescent inorganic materials have been studied extensively as fluorescent sensors, and diagnostic and bioimaging tools. The assessment of photoluminescence (PL) properties of selenium nanoparticles (Se NPs), especially mycosynthesized Se NPs, is still in its infancy. Herein, we have biosynthesized highly dispersed fluorescent Se NPs (42 nm) using endophytic fungus Fusarium oxysporum, and fully characterized them using sophisticated instruments like TEM, XRD, UV-Vis spectrophotometer, FTIR, and PL spectrometer. To determine the therapeutic efficacy and side effect profiles, these crystalline Se NPs were radiolabeled with technetium-99m (^99mTc) and their biodistribution and renal clearance times were investigated in the normal Wister rat. The results showed that these Se NPs may be useful for targeting the lungs and liver dysfunction as significant accumulation of these NPs was observed in the liver (approx. 19.47 ± 4%) and lungs (at 6 ± 1%) after 10 min of post-injection. Quick circulation and the presence of Se NPs in kidney (3.8 ± 2%) also suggested the easy excretion of these NPs from the body through urinary tract. Furthermore, the antioxidant activity of Se NPs (IC₅₀, 159.5 μg/mL) has been investigated using DPPH free radical scavenging assay with scavenging efficacy of 80.4% where ascorbic acid (IC₅₀, 5.6 μg/mL) was used as a positive control. Additionally, the microscopic study of the inhibition zone encircled around Se NPs confirmed their strong antifungal and antisporulant activity against the black fungus Aspergillus niger.

Keywords: Antifungal; Aspergillus niger; Biodistribution; Fluorescent; Fusarium oxysporum; Selenium nanoparticles.

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