Preparation and characterization of garlic polysaccharide-Zn (II) complexes and their bioactivities as a zinc supplement in Zn-deficient mice

Xinyan Bai; Zhichang Qiu; Zhenjia Zheng; Shuoshuo Song; Renjie Zhao; Xiaoming Lu; Xuguang Qiao

doi:10.1016/j.fochx.2022.100361

Preparation and characterization of garlic polysaccharide-Zn (II) complexes and their bioactivities as a zinc supplement in Zn-deficient mice

Food Chem X. 2022 Jun 14:15:100361. doi: 10.1016/j.fochx.2022.100361. eCollection 2022 Oct 30.

Authors

Xinyan Bai¹, Zhichang Qiu¹, Zhenjia Zheng¹, Shuoshuo Song¹, Renjie Zhao¹, Xiaoming Lu¹, Xuguang Qiao¹

Affiliation

¹ Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an, Shandong 271018, PR China.

Abstract

This study explored the potential of garlic polysaccharides (GPs) as a carrier for synthesizing GP-Zn (II) complexes to supplement Zn. According to the response surface analysis, the optimal preparation conditions were: mass ratio of GPs to Zn²⁺ 1:0.21, temperature 53 °C, pH 5.9 and time 148.75 min, with the maximum chelation rate of 90.11%. The chelation of GPs and Zn²⁺ involved O-H/C-O/O-C-O groups, increased crystallinity and altered absorption peaks of circular dichroism spectra, with a higher thermal stability, particle size and negative zeta potential. Compared with inorganic zinc salts, supplementation of GP-Zn (II) complexes showed enhance zinc supplementation effects in Zn-deficient mice model: increased body weight, organ index and Zn (II) levels in serum and liver, enhanced Superoxidedismutase (SOD) activity and alkaline phosphatase activity, decreased NO content and Malondialdehyde (MDA) content and improved colon and testicular morphology. Therefore, GP-Zn (II) complex can be used as a potential zinc supplement for Zn-deficient individuals.

Keywords: Polysaccharide-Zn (II) complex; Preparation; Structural characterization; Zinc supplement.