Hypoxia Modulates Melanoma Cells Proliferation and Apoptosis via miRNA-210/ISCU/ROS Signaling

Bull Exp Biol Med. 2022 Sep;173(5):645-650. doi: 10.1007/s10517-022-05605-0. Epub 2022 Oct 10.

Abstract

In this study, luciferase reporter assay was used to establish the relationship between miR-210 and ISCU. This research was performed on both cell lines (A2058, G361, and 293T) and tissue samples. We found that miR-210 was upregulated in hypoxia and was elevated in melanoma in comparison with adjacent normal tissues. Expression of ISCU protein was decreased in melanoma tissues, and ISCU gene is a direct target of miR-210. ISCU knockdown with miR-210 enhanced ROS production. The results of our study showed that miR-210/ISCU/ROS axis can serve as a novel therapeutic target in melanoma.

Keywords: ISCU; apoptosis; hypoxia; melanoma cell lines; miR-210.

MeSH terms

  • Apoptosis / genetics
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Hypoxia / genetics
  • Iron-Sulfur Proteins* / genetics
  • Melanoma* / genetics
  • Melanoma* / metabolism
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Reactive Oxygen Species / metabolism
  • Sulfonylurea Compounds

Substances

  • ISCU protein, human
  • Iron-Sulfur Proteins
  • MIRN210 microRNA, human
  • MicroRNAs
  • Reactive Oxygen Species
  • Sulfonylurea Compounds
  • sulofenur