Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables

Le Zhang; Yiliu Zheng; Hua Shao; Ming Xiao; Jianchun Sun; Maojun Jin; Fen Jin; Jing Wang; A M Abd El-Aty; Yongxin She

doi:10.3389/fnut.2022.957745

Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables

Front Nutr. 2022 Sep 20:9:957745. doi: 10.3389/fnut.2022.957745. eCollection 2022.

Authors

Le Zhang¹, Yiliu Zheng¹, Hua Shao¹, Ming Xiao², Jianchun Sun³, Maojun Jin¹, Fen Jin¹, Jing Wang¹, A M Abd El-Aty^{4

5

6}, Yongxin She¹

Affiliations

¹ Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.
² Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China.
³ Inspection and Testing Center of Agricultural Products of Tibetan Autonomous Region, Lhasa, China.
⁴ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China.
⁵ Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
⁶ Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey.

Abstract

Fenvalerate residues in fruits and vegetables may result in biological immune system disorders. Current sensor detection methods are harsh due to the shortcomings of antibody preparation and preservation conditions. Therefore, developing a recognition material with strong specificity, good stability, and low cost is of practical significance in designing a sensitive, simple, and rapid method. This study used precipitation polymerization to synthesize molecularly imprinted polymers (MIPs). The MIP was prepared into a fiber membrane using the electrostatic spinning method. After that, the fenvalerate hapten-mouse IgG-Eu fluorescent probe was synthesized, and the side flow chromatography strip was constructed to determine fenvalerate in vegetables using the immunocompetition method. The results showed that the adsorption capacity of MIP to fenvalerate was 3.65, and the adsorption capacity on MIPFM (an electrospinning membrane containing the fenvalerate MIPs) was five times that of free MIP. The test strip showed good linearity with R ² = 0.9761 within the range of 50 μg/L-1,000 μg/L. In conclusion, substituting fenvalerate monoclonal antibodies with a molecularly imprinted electrospinning membrane is ideal for rapid onsite detection of pyrethroids.

Keywords: electrospinning membrane; fenvalerate; fluorescence; molecularly imprinted polymer; test strip.