Analysis of genetic diversity among Chinese Cyclocybe chaxingu strains using ISSR and SRAP markers

Jin-Hao Liu; Fang-Hui Ding; Hai-Yan Song; Ming-Hui Chen; Dian-Ming Hu

doi:10.7717/peerj.14037

Analysis of genetic diversity among Chinese Cyclocybe chaxingu strains using ISSR and SRAP markers

PeerJ. 2022 Sep 29:10:e14037. doi: 10.7717/peerj.14037. eCollection 2022.

Authors

Jin-Hao Liu^{1

2

3}, Fang-Hui Ding², Hai-Yan Song^{1

4}, Ming-Hui Chen^{1

2

3}, Dian-Ming Hu^{1

2

3}

Affiliations

¹ Jiangxi Agricultural University, Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, Nanchang, China.
² Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang, China.
³ Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, and College of Bioscience and Bioengineering, Nanchang, China.
⁴ Ministry of Education of the P.R. China, Key Laboratory of Crop Physiology, Ecology and Genetic Breeding (Jiangxi Agricultural University), Nanchang, China.

Abstract

Background: Cyclocybe chaxingu is an edible and medicinal fungal species commonly cultivated in China. The major problems currently facing by growers of C. chaxingu is the random labeling of strains and strains aging and degeneration. Therefore, an evaluation of genetic diversity is essential for the conservation and reproducing programs of this species.

Methods: In the present study, 24 widely cultivated strains were collected from the main producing areas of China, and the genetic diversity analysis was performed. DNA polymorphism among these Chinese C. chaxingu strains was analyzed using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers.

Results: Eight ISSR primers amplified a total of 75 DNA fragments of which 61 (81.33%) were polymorphic. Fifteen SRAP primer combinations amplified 166 fragments of which 132 (79.52%) were polymorphic. Cluster analysis showed that the C. chaxnigu strains fall into five groups with a genetic distance values ranging from 0.06 to 0.60 by ISSR analysis, while the SRAP analysis divided the test strains into four groups within the range of genetic distance from 0.03 to 0.57. The results of the present study reveal a high level of genetic diversity among the widely cultivated C. chaxingu strains.

Keywords: Cyclocybe chaxingu; Genetic diversity; Germplasm resource; Molecular markers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales*
East Asian People
Genetic Variation* / genetics
Humans
Microsatellite Repeats / genetics
Polymorphism, Genetic / genetics

Grants and funding

Funds for research were provided by the National Natural Science Foundation of China (NSFC 32070023, NSFC 32060014), the Key Projects of Youth Fund of Jiangxi Science, Technology Department of China (20192ACBL21017), the Natural Science Foundation of Education Department of Jiangxi Province of China (GJJ190168) and the earmarked fund for jiangxi Agriculture Research System. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.