Profiling of phospholipid classes and molecular species in human milk, bovine milk, and goat milk by UHPLC-Q-TOF-MS

Yue Sun; Songfan Tian; Muhammad Hussain; Shuang Lin; Yue Pan; Xiaodong Li; Lu Liu; Xinyu Lu; Chunmei Li; Youbin Leng; Shilong Jiang

doi:10.1016/j.foodres.2022.111872

Profiling of phospholipid classes and molecular species in human milk, bovine milk, and goat milk by UHPLC-Q-TOF-MS

Food Res Int. 2022 Nov:161:111872. doi: 10.1016/j.foodres.2022.111872. Epub 2022 Aug 27.

Authors

Yue Sun¹, Songfan Tian¹, Muhammad Hussain¹, Shuang Lin¹, Yue Pan¹, Xiaodong Li², Lu Liu³, Xinyu Lu¹, Chunmei Li⁴, Youbin Leng⁵, Shilong Jiang⁵

Affiliations

¹ Food College, Northeast Agricultural University, No.600 Changjiang St., Xiangfang Dist, 150030 Harbin, China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, No. 600 Changjiang St., Xiangfang Dist, 150030 Harbin, China.
² Food College, Northeast Agricultural University, No.600 Changjiang St., Xiangfang Dist, 150030 Harbin, China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, No. 600 Changjiang St., Xiangfang Dist, 150030 Harbin, China. Electronic address: hrblxd@163.com.
³ Food College, Northeast Agricultural University, No.600 Changjiang St., Xiangfang Dist, 150030 Harbin, China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, No. 600 Changjiang St., Xiangfang Dist, 150030 Harbin, China. Electronic address: liulu89824@163.com.
⁴ Heilongjiang Institute of Green Food Science, 150028 Harbin, China.
⁵ Heilongjiang Feihe Dairy Co., Ltd., 100015 Beijing, China.

PMID: 36192991
DOI: 10.1016/j.foodres.2022.111872

Abstract

This study aimed to identify differences of lipidome and fatty acid composition among human, bovine and caprine milk fat globule membrane (MFGM) using a lipidomics approach. In total, 11 lipid classes were determined, including PC, PE, SM, PG, PI, PS, Cer, PA, HexCer, Hex₂Cer and CL. Using positive and negative ionization modes, 73 and 156 common significantly differential lipid species were identified in three typical MFGM. The oleic acid (C18:1) was the dominant fatty acid in human (28.29 ± 0.04 g/100 g) and caprine (35.71 ± 0.02 g/100 g) MFGM, whereas Palmitic acid (C16:0, 29.13 ± 0.07 g/100 g) was the dominant fatty acid in bovine MFGM. In addition, there was no significant difference in long chain fatty acids between human MFGM and caprine milk. Overall, the results provide essential insights into differential lipids characterization in MFGM, along with a theoretical basis for the design of new infant formula.

Keywords: Fatty acid; Human milk; Milk fat globule membrane; Phospholipid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, High Pressure Liquid
Fatty Acids
Goats*
Humans
Infant
Milk, Human*
Oleic Acids
Palmitic Acids
Phospholipids

Substances

Fatty Acids
Oleic Acids
Palmitic Acids
Phospholipids