Insight into the microbial interactions of Anammox and heterotrophic bacteria in different granular sludge systems: effect of size distribution and available organic carbon source

Jiarui Fan; Rui Du; Qingtao Liu; Cong Li; Yongzhen Peng

doi:10.1016/j.biortech.2022.128055

Insight into the microbial interactions of Anammox and heterotrophic bacteria in different granular sludge systems: effect of size distribution and available organic carbon source

Bioresour Technol. 2022 Nov:364:128055. doi: 10.1016/j.biortech.2022.128055. Epub 2022 Sep 30.

Authors

Jiarui Fan¹, Rui Du², Qingtao Liu¹, Cong Li¹, Yongzhen Peng¹

Affiliations

¹ National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
² National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: durui@bjut.edu.cn.

PMID: 36191754
DOI: 10.1016/j.biortech.2022.128055

Abstract

Microbial interactions between Anammox and heterotrophic bacteria in different granule distributions in an Anammox system (AMX) and partial denitrification coupled with Anammox system (PDA) were analyzed in this paper. Candidatus Brocadia was the main Anammox microorganism in granules of 1.0 > d > 0.5 mm with the highest abundance of 21.5 % in AMX, significantly higher than the maximum proportion of 2.3 % in PDA sludge > 2.0 mm. However, the total nitrogen (TN) removal of 77.9 % in AMX was lower than PDA (94.0 %) because of the excessive NO₃^--N generated by nitrite-oxidizing bacteria (NOB). Anammox activity could be stimulated by heterotrophs via simple organic carbon, which decreased with the increasing size of sludge in AMX but increased in PDA. This highlighted that regulation of the distribution of sludge size and organic carbon source had an essential effect on efficient nitrogen removal of Anammox technology.

Keywords: Anammox; Granule sludge; Microbial community; Partial denitrification; Particle size distribution.