Establishment of lactate-metabolism-related signature to predict prognosis and immunotherapy response in patients with colon adenocarcinoma

Zhengrong Zou; Yongjie Chai; Qi Li; Xuan Lin; Qingfang He; Qiusheng Xiong

doi:10.3389/fonc.2022.958221

Establishment of lactate-metabolism-related signature to predict prognosis and immunotherapy response in patients with colon adenocarcinoma

Front Oncol. 2022 Sep 14:12:958221. doi: 10.3389/fonc.2022.958221. eCollection 2022.

Authors

Zhengrong Zou¹, Yongjie Chai², Qi Li³, Xuan Lin¹, Qingfang He⁴, Qiusheng Xiong⁴

Affiliations

¹ Department of Emergency Trauma Center, The First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
² Department of Anorectal Surgery, Zhucheng People's Hospital, Zhucheng, China.
³ Basic Medicine Department of Chuxiong Medical and Pharmaceutical College, Chuxiong, China.
⁴ Department of General Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, China.

Abstract

The outcome of colon adenocarcinoma (COAD) patients remains dismal, and lactate metabolism has been characterized to promote tumor development and immune evasion. Based on the above background, it is worthwhile to explore novel prognostic and therapeutic biomarkers for COAD patients from the aspect of lactate metabolism. Above all, 228 available lactate-metabolism-related genes (LMRGs) were acquired, and the landscape of copy number variation and the expression difference of mRNA levels between colon normal and tumor samples were investigated among these LMRGs. Importantly, eight overall survival (OS)-involved LMRGs were then distinguished by means of univariate Cox regression analysis in both GSE40967 and TCGA-COAD data sets. Subsequently, prognostic risk scores were established, integrating seven OS-related LMRGs by LASSO Cox regression analysis in the GSE40967 set, and then verified in the TCGA-COAD cohort. From the comprehensive analyses, COAD patients with high risk had comparatively more inferior survival probability in all populations of the study, and they tended to have more severe clinicopathological features with the risk score increasing. Moreover, by integrating age, AJCC T and pathological stage, and risk score, we constructed a prognostic nomogram that demonstrated great prediction effectiveness for OS of COAD patients. Furthermore, the potential effect of various risk score on tumor immune was assessed from enrichment of immune-related pathways, tumor-infiltrating immune cells, and expression levels of immune checkpoints separately. We could draw a conclusion that COAD patients with higher lactate-metabolism-related risk scores may acquire an immunosuppressive tumor microenvironment, which subsequently led to immune escapes and poor prognoses. Conclusively, all findings in the present study illustrate a great prognostic value of the lactate-metabolism-related risk signature, providing more in-depth insights into the indispensable function of lactate metabolism in prognosis and tumor immunity of COAD.

Keywords: colon adenocarcinoma; immunotherapy; lactate metabolism; risk signature; tumor immune microenvironment.