Dihydroflavonoid glycosides from Viscum album and their inhibitory effects on hepatic lipid accumulation and target identification

Guo-Zhu Su; Shang-Yi Wang; Xiu-Ying Yang; Zora Dajić Stevanović; Na Li; Nikola Tanić; Nebojsa Arsenijević; Shi-Shan Yu; Yong Li

doi:10.1016/j.phytochem.2022.113458

Dihydroflavonoid glycosides from Viscum album and their inhibitory effects on hepatic lipid accumulation and target identification

Phytochemistry. 2022 Dec:204:113458. doi: 10.1016/j.phytochem.2022.113458. Epub 2022 Sep 28.

Authors

Guo-Zhu Su¹, Shang-Yi Wang¹, Xiu-Ying Yang¹, Zora Dajić Stevanović², Na Li¹, Nikola Tanić³, Nebojsa Arsenijević⁴, Shi-Shan Yu⁵, Yong Li⁶

Affiliations

¹ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.
² Faculty of Agriculture, University of Belgrade, Belgrade, 11080, Serbia.
³ Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade, Belgrade, 11060, Serbia.
⁴ Faculty of Medical Sciences, University of Kragujevac, Kragujevac, 34000, Serbia.
⁵ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address: yushishan@imm.ac.cn.
⁶ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address: liyong@imm.ac.cn.

PMID: 36181860
DOI: 10.1016/j.phytochem.2022.113458

Abstract

Five undescribed dihydroflavonoid glycoside derivatives, namely albvisosides A‒E, together with two known compounds were isolated from the roots and stem leaves of Viscum album L. var. album. (European mistletoe). Their structures were determined by HRESIMS, 1D and 2D NMR, and ECD analysis. Albvisoside B exhibits significant inhibitory effect on hepatic lipid accumulation in HepG2 cells at very low concentrations (EC₅₀: 0.7 nM). Using proteome integral solubility alteration assay, the direct targets or downstream effectors of albvisoside B were elucidated. As a result, 97 proteins were identified based on ligand-induced alterations in the protein thermal stability. Bioinformatics analysis indicated that albvisoside B primarily ameliorated oleic acid-induced lipid accumulation by regulating the selenoamino acids metabolism signaling pathway. RPL3, ADAM17, and RPL14 were likely to be involved in mediating the lipid-lowering effect of albvisoside B.

Keywords: Album; Dihydroflavonoid glycoside; Lipid accumulation; Loranthaceae; PISA; Viscum album var.