Novel mesoporous silica surface loaded gold nanocomposites SERS aptasensor for sensitive detection of zearalenone

Zhiming Guo; Lingbo Gao; Limei Yin; Muhammad Arslan; Hesham R El-Seedi; Xiaobo Zou

doi:10.1016/j.foodchem.2022.134384

Novel mesoporous silica surface loaded gold nanocomposites SERS aptasensor for sensitive detection of zearalenone

Food Chem. 2023 Mar 1:403:134384. doi: 10.1016/j.foodchem.2022.134384. Epub 2022 Sep 24.

Authors

Zhiming Guo¹, Lingbo Gao², Limei Yin², Muhammad Arslan², Hesham R El-Seedi³, Xiaobo Zou⁴

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing (Jiangsu University), Jiangsu Education Department, Zhenjiang 212013, China. Electronic address: guozhiming@ujs.edu.cn.
² School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
³ Pharmacognosy Group, Department of Pharmaceutical Biosciences, BMC, Uppsala University, Box 591, SE 751 24 Uppsala, Sweden; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China.
⁴ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China.

PMID: 36179642
DOI: 10.1016/j.foodchem.2022.134384

Abstract

Mycotoxin contamination is a severe threat to global food security, thus fast and effective detection of mycotoxins is of great significance. Herein, mesoporous silica surface loaded gold nanocomposites (MSN-Rh6G-AuNPs) were prepared as surface-enhanced Raman scattering (SERS) substrate, and the SERS aptasensor (MSN-Rh6G-AuNPs@apt) was further obtained by aptamer functionalization which can realize the quantitative and sensitive detection of zearalenone (ZEN). The small nanogaps between AuNPs made MSN-Rh6G-AuNPs present strong SERS performance under excitation light irradiation, while the aptamer performed the functions of ZEN recognition and Raman signal masking. The acquired results revealed that the SERS intensity at 1508 cm^-1 had a good linear relationship with ZEN concentration of 3-200 ng/mL and the limit of detection (LOD) was calculated to be 0.0064 ng/mL. In addition, the designed SERS aptasensor was successfully applied to the detection of ZEN in corn, indicating great potential in practical implications.

Keywords: Aptamer functionalization; Food safety; Nanocomposites; Specific detection; Surface-enhanced Raman spectroscopy; Zearalenone.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Gold
Limit of Detection
Metal Nanoparticles*
Nanocomposites*
Silicon Dioxide
Spectrum Analysis, Raman / methods
Zearalenone* / analysis

Substances

Gold
Zearalenone
Silicon Dioxide
Aptamers, Nucleotide