Using of eosin Y as a facile fluorescence probe in alogliptin estimation: Application to tablet dosage forms and content uniformity testing

Mohamed M Salim; Aya A Marie; Amira H Kamal; Sherin F Hammad; Mahmoud M Elkhoudary

doi:10.1016/j.saa.2022.121919

Using of eosin Y as a facile fluorescence probe in alogliptin estimation: Application to tablet dosage forms and content uniformity testing

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 15:285:121919. doi: 10.1016/j.saa.2022.121919. Epub 2022 Sep 24.

Authors

Mohamed M Salim¹, Aya A Marie², Amira H Kamal³, Sherin F Hammad³, Mahmoud M Elkhoudary⁴

Affiliations

¹ Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34517, Egypt.
² Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34517, Egypt; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt.
³ Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt.
⁴ Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34517, Egypt. Electronic address: mmasalim@mans.edu.eg.

PMID: 36179566
DOI: 10.1016/j.saa.2022.121919

Abstract

New simple sensitive and reliable spectrofluorimetric approach was established for the determination of the antidiabetic drug; Alogliptin (ALG) in its pure and tablet forms. The developed approach is depended on the suppressive action of ALG on the eosin Y native fluorescence. The quenching action of ALG on the eosin Y native fluorescence was measured at acidic medium pH: 3.5, emission wavelength 541 nm (λ_ex. 260 nm). The relative fluorescence intensity (RFI) was measured, and it was directly proportional to ALG concentration in the concentration range of (15-110) μg/mL. The developed and optimized approach was entirely validated regarding to ICH guidelines. The developed method application was successfully extended for ALG content uniformity test (CU). The distribution fraction (DF), rate constants (K), and free energy changes (ΔG°) were calculated. The results obtained were compared to that of the published spectrophotometric one.

Keywords: Alogliptin; Content Uniformity Test; Dosage Form; Eosin Y; Spectrofluorimetry.

MeSH terms

Eosine Yellowish-(YS)
Fluorescent Dyes*
Spectrometry, Fluorescence / methods
Tablets

Substances

Eosine Yellowish-(YS)
Fluorescent Dyes
alogliptin
Tablets