Regulation of Gene Expression Using dCas9-SunTag Platforms

Sumiyo Morita; Takuro Horii; Izuho Hatada

doi:10.1007/978-1-0716-2724-2_13

Regulation of Gene Expression Using dCas9-SunTag Platforms

Methods Mol Biol. 2023:2577:189-195. doi: 10.1007/978-1-0716-2724-2_13.

Authors

Sumiyo Morita¹, Takuro Horii¹, Izuho Hatada²

Affiliations

¹ Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan.
² Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan. hatada@gunma-u.ac.jp.

PMID: 36173574
DOI: 10.1007/978-1-0716-2724-2_13

Abstract

Regulating gene expression is important for basic research and therapeutic applications. The epigenome is a record of genetic modifications such as DNA methylation and histone modifications, and epigenetic changes can play a key role in modifying gene expression. With the advent of genome editing technologies, it has become possible to manipulate the epigenome of specific genomic regions to control gene expression. In particular, CRISPR-Cas9 systems have been used widely for epigenome editing due to their high efficiency, versatility, specificity, and ease of use. Here, we describe a protocol for the upregulation of specific genes using the dCas9-SunTag system.

Keywords: DNA methylation; Epigenetic editing; SunTag; TET1; VP64; dCas9.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
DNA Methylation
Epigenesis, Genetic
Gene Editing* / methods
Gene Expression Regulation
Transcriptional Activation