Antimicrobial activity and safety features assessment of Weissella spp. from environmental sources

Imene Fhoula; Mohamed Boumaiza; Ghassan Tayh; Amel Rehaiem; Naouel Klibi; Imene-Hadda Ouzari

doi:10.1002/fsn3.2885

Antimicrobial activity and safety features assessment of Weissella spp. from environmental sources

Food Sci Nutr. 2022 Apr 15;10(9):2896-2910. doi: 10.1002/fsn3.2885. eCollection 2022 Sep.

Authors

Imene Fhoula¹, Mohamed Boumaiza¹, Ghassan Tayh^{1

2}, Amel Rehaiem^{3

4}, Naouel Klibi¹, Imene-Hadda Ouzari¹

Affiliations

¹ Laboratoire Microorganismes et Biomolécules Actives (LR03ES03) Faculté des Sciences de Tunis Université Tunis El Manar Tunis Tunisia.
² Service de Microbiologie et d'Immunologie Ecole Nationale de Médecine Vétérinaire Université Manouba Sidi Thabet Tunisia.
³ Faculty of Medicine of Tunis Research Laboratory "Antimicrobial Resistance" LR99ES09 University of Tunis El Manar Tunis Tunisia.
⁴ Laboratory of Microbiology Charles Nicolle Hospital Tunis Tunisia.

Abstract

Weissella strains have been reported to be useful in biotechnological and probiotic determinations, and some of them are considered opportunistic pathogens. Given the widespread interest about antimicrobial susceptibilities, transmission of resistances, and virulence factors, there is little research available on such topics for Weissella. The aim of this study was to assess the safety aspects and antimicrobial potential of 54 Weissella spp. strains from different environmental sources. Antibiotic susceptibility, hemolytic activity, horizontal transfer, and antibacterial activity were studied, as well as the detection of biogenic amine BA production on decarboxylase medium and PCR was performed. All the strains were nonhemolytic and sensitive to chloramphenicol and ampicillin. Several strains were classified as resistant to fusidic acid, and very low resistance rates were detected to ciprofloxacin, tetracycline, streptomycin, lincomycin, erythromycin, and rifampicin, although all strains had intrinsic resistance to vancomycin, nalidixic acid, kanamycin, and teicoplanin. Two BA-producing strains (W. halotolerans FAS30 and FAS29) exhibited tyrosine decarboxylase activity, and just one W. confusa FS077 produced both tyramine and histamine, and their genetic determinants were identified. Ornithine decarboxylase/odc gene was found in 16 of the Weissella strains, although 3 of them synthesize putrescine. Interestingly, eight strains with good properties displayed antibacterial activity. Conjugation frequencies of erythromycin from Bacillus to Weissella spp. varied in the average of 3 × 10^-9 transconjugants/recipient. However, no tetracycline-resistant transconjugant was obtained with Enterococcus faecalis JH2-2 as recipient. The obtained results support the safe status of Weissella strains, derived from environmental sources, when used as probiotics in animal feed.

Keywords: Weissella; antibacterial; antibiotic resistance; biogenic amines; environment; safety.