Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling

Gakuya Takamatsu; Yoko Manome; Jun-Seok Lee; Kanako Toyama; Tomoko Hayakawa; Chikako Hara-Miyauchi; Minami Hasegawa-Ogawa; Chiaki Katagiri; Tsuyoshi Kondo; Hirotaka James Okano; Masayuki Matsushita

doi:10.1016/j.scr.2022.102915

Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling

Stem Cell Res. 2022 Oct:64:102915. doi: 10.1016/j.scr.2022.102915. Epub 2022 Sep 14.

Affiliations

¹ Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Neuropsychiatry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
² Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
³ Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Advanced Medical Research Center, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
⁴ Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Division of Clinical Pharmacology, Department of Pharmacology, Jichi Medical University, Tochigi, Japan.
⁵ Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Center for Brain Integration Research, Tokyo Medical and Dental University, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kanagawa, Japan.
⁶ Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Synbiotics, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan.
⁷ Department of Neuropsychiatry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
⁸ Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan. Electronic address: masayuki@med.u-ryukyu.ac.jp.

PMID: 36166871
DOI: 10.1016/j.scr.2022.102915

Abstract

Induced pluripotent stem cells (iPSCs) obtained from genetically characterized patients benefit the biological study of bipolar disorder (BD). Here, we present iPSC lines from three-generation patients with BD and recurrent depressive disorder (RDD) and a healthy control sibling in a family. All patients shared the specified haplotype in the 1p36-35, previously reported as the susceptibility locus of mood disorders. iPSCs were generated with the reprogramming factors OTC3/4, l-MYC, LIN28, SOX2, KLF4, and p53 shRNA through non-integrated episomal vectors. All iPSC lines strongly expressed pluripotency markers and proved the ability to differentiate into three germ lineages in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bipolar Disorder* / genetics
Depressive Disorder*
Haplotypes / genetics
Humans
Induced Pluripotent Stem Cells*
RNA, Small Interfering
Siblings
Tumor Suppressor Protein p53

Substances

RNA, Small Interfering
Tumor Suppressor Protein p53