Rapid enrichment of anammox bacteria and transformation to partial denitrification/anammox with nitrification/denitrification sludge

Abstract

The stable nitrite (NO₂^--N) generation and rapid startup of anammox-based process are the main bottlenecks hindering its application in mainstream municipal wastewater treatment. In this study, a Partial-Denitrification (PD) system reducing nitrate (NO₃^--N) to NO₂^--N was rapidly developed within 40 days, using the nitrification/denitrification sludge from wastewater treatment plant. The NO₃^--N to NO₂^--N transformation ratios achieved 80.6 %. Significantly, a fast self-enrichment of anammox bacteria in this system was subsequently obtained, resulting in the successful transformation to an efficient PD/Anammox (PD/A) process after 79-day operation. The total nitrogen removal efficiency increased from 12.4 % to 90.0 % with influent ammonia and nitrate of 45.9 mg N/L and 62.2 mg N/L, corresponding to the anammox activity significantly increasing to 6.0 mgNH₄⁺-N/g VSS/h without seeding anammox sludge. Abundance of anammox increased from 6.7 × 10⁸ to 2.0 × 10¹¹ copies/g dry sludge. High-throughput sequencing results showed that Candidatus Brocadia was the only known anammox genus and accounted for 1.08 % during the PD/A stage. Functional bacteria for PD, assumed to be the Thauera, was enriched from 1.99 % to 60.06 % but decreased to 32.49 % during the improvement of anammox activity. It demonstrated that the PD system with stable NO₂^--N accumulation enabled a rapid self-enrichment of anammox bacteria and sufficient nitrogen removal with ordinary nitrification/denitrification sludge. This provides new insights into the scaling application of anammox by integrating PD with shortened startup periods and improved TN removal efficiency.

Keywords: Functional bacteria; Mainstream municipal wastewater treatment; Nitrite accumulation; Partial denitrification; Self-enrichment of anammox bacteria.