A method to test antibody cross-reactivity toward animal antigens for flow cytometry

Cytometry A. 2023 May;103(5):455-457. doi: 10.1002/cyto.a.24691. Epub 2022 Sep 26.

Abstract

The availability of cross-reacting antibodies and/or of antibodies working in flow cytometry is a major issue in the veterinary field. One of the main problems is the availability of certain positive controls. With this brief communication, we report an method to quickly screen a wide number of products without the need to look for positive biological samples. We propose this approach as a first step to select the best antibodies to test on biological specimens.

Keywords: PD-L1; cross-reactivity; dog; microbeads; veterinary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies*
  • Antigens*
  • Flow Cytometry

Substances

  • Antibodies
  • Antigens