The chick embryo chorioallantoic membrane (CAM), an extensively vascularized extraembryonic membrane, has been widely used to study several aspects of tumor development including tumor-induced angiogenesis, tumor cell proliferation, and metastasis. Based on the tumor cell/CAM system, we focused here on the identification and quantification of cancer stem cells. We validated the CAM model as a suitable model to evaluate stem cell activity in a given mixed cell population.
Keywords: Cancer stem cells; Chorioallantoic membrane; Limiting dilution assays.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.