Beneficial effects of whey protein peptides on muscle loss in aging mice models

Xin Wu; Xiaochen Yu; Na Zhu; Meihong Xu; Yong Li

doi:10.3389/fnut.2022.897821

Beneficial effects of whey protein peptides on muscle loss in aging mice models

Front Nutr. 2022 Sep 9:9:897821. doi: 10.3389/fnut.2022.897821. eCollection 2022.

Authors

Xin Wu^{1

2}, Xiaochen Yu¹, Na Zhu¹, Meihong Xu¹, Yong Li¹

Affiliations

¹ Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, China.
² Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Peking University Research Center on Aging, Peking University, Beijing, China.

Abstract

Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects of whey protein peptides (WPPs) on sarcopenia in d-gal-induced aging mice. A total of 72 SPF male C57BL/6N mice were used in this study. Sixty mice were modeled by injected intraperitoneally with d-gal (100 mg/kg body weight for 6 weeks), and the other 12 mice were used as control, and injected with the same amount of normal saline. After 6 weeks, the modeled mice were randomly divided into the model control group, whey protein group (1.5 g/kg^*bw), and three WPPs intervention groups (0.3 g/kg^*bw, 1.5 g/kg^*bw, 3.0 g/kg^*bw), according to serum malondialdehyde (MDA) level. The test samples were orally given to mice by daily garaged. During the 30 days intervention period, the model control group, whey protein group, and WPPs group continued receiving intraperitoneal injections of d-gal, whereas the control group continued receiving intraperitoneal injections of normal saline. The results showed that WPPs could significantly improve the grip strength of aged mice. WPPs could significantly increase lean mass of aged mice and increase muscle weight of gastrocnemius and extensor digitorum longus. WPPs could significantly increase the level of insulin-like growth factor-1 (IGF-1) and reduce level of interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-α) in serum. WPPs could affect the muscle fiber size in d-gal-induced aging mice. Its specific mechanism may be related to the activation of IGF-1/Akt/mTOR protein synthesis signaling pathway and reduction of the level of inflammation. These results indicate that WPPs can improve aging-related sarcopenia. Compared with whey protein, WPPs supplement seems a better form for sarcopenia.

Keywords: age-related; d-galactose (d-gal); muscle loss; protein synthesis; sarcopenia; skeletal muscles; whey protein peptides.