Indigenous rhizosphere microbial community characteristics of the phytostabilizer Athyrium wardii (Hook.) grown in a Pb/Zn mine tailing

Abstract

Plant rhizosphere microbiome usually changes dramatically in adaptation to the mine environment to endure high heavy metal concentration, which in turn improves the process of revegetation and phytostabilization of mine tailing and deserves deep investigation. A field study was conducted to investigate the indigenous microbial community of a mining ecotype (ME) of the phytostabilizer Athyrium wardii (Hook.) grown in a Pb/Zn mine tailing and a corresponding non-mining ecotype (NME) grown in an uncontaminated adjacent site. Our study found a slight difference in microbial α-diversity between the ME and NME, and no significant difference between the rhizosphere and bulk soil. Both bacterial and fungal community compositions differed between the ME and NME, for which the differences were mainly driven by pH and metal contaminants. The ME harbored a unique microbial community in the rhizosphere soils different from the bulk soil and NME counterparts. The dominant phyla in the ME rhizosphere were Proteobacteria, Chloroflexi, Actinobacteria, Nitrospirae, and Ascomycota. Several genera from Proteobacteria, Acidobacteria, and Ascomycota were more abundant in the ME rhizosphere than in the NME rhizosphere. Network analysis revealed that keystone taxa were different in the two sites. Some keystone taxa from Gemmatimonadaceae, and Burkholderiaceae and Ascomycota played a critical role in microbial interactions within the mine tailing network. The unique microbial community with high tolerance in the rhizosphere soils of ME may show great benefit for plant growth and metal tolerance of the ME and thereby contributing to the process of revegetation and phytostabilization of mine tailings.

Keywords: Co-occurrence network; Heavy metal; Phytostabilization; Revegetation; Rhizosphere bacterial and fungal community.