DNA Methylation Analysis of Cnr1 Gene Promoter

Claudio D'Addario; Mariangela Pucci

doi:10.1007/978-1-0716-2728-0_31

DNA Methylation Analysis of Cnr1 Gene Promoter

Methods Mol Biol. 2023:2576:373-384. doi: 10.1007/978-1-0716-2728-0_31.

Authors

Claudio D'Addario¹, Mariangela Pucci²

Affiliations

¹ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
² Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. mpucci@unite.it.

PMID: 36152203
DOI: 10.1007/978-1-0716-2728-0_31

Abstract

DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing.

Keywords: DNA extraction; DNA methylation; Methylation-specific PCR (MSP); Pyrosequencing; Type-1 cannabinoid receptor.

MeSH terms

DNA Methylation*
DNA*
Humans
Polymerase Chain Reaction / methods
Promoter Regions, Genetic
Receptor, Cannabinoid, CB1 / genetics
Sequence Analysis, DNA / methods

Substances

CNR1 protein, human
Receptor, Cannabinoid, CB1
DNA