Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK αVβ6 Cell Line for Senecavirus A Research

Jessica Mason; Victoria Primavera; Lauren Martignette; Benjamin Clark; Jose Barrera; Janine Simmons; William Hurtle; John G Neilan; Michael Puckette

doi:10.3390/v14091875

Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK α_Vβ₆ Cell Line for Senecavirus A Research

Viruses. 2022 Aug 25;14(9):1875. doi: 10.3390/v14091875.

Authors

Jessica Mason¹, Victoria Primavera¹, Lauren Martignette¹, Benjamin Clark², Jose Barrera¹, Janine Simmons¹, William Hurtle³, John G Neilan³, Michael Puckette³

Affiliations

¹ SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA.
² U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY 11944, USA.
³ U.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA.

Abstract

Senecavirus A (SVA) is a member of the family Picornaviridae and enzootic in domestic swine. SVA can induce vesicular lesions that are clinically indistinguishable from Foot-and-mouth disease, a major cause of global trade barriers and agricultural productivity losses worldwide. The LF-BK α_Vβ₆ cell line is a porcine-derived cell line transformed to stably express an α_Vβ₆ bovine integrin and primarily used for enhanced propagation of Foot-and-mouth disease virus (FMDV). Due to the high biosecurity requirements for working with FMDV, SVA has been considered as a surrogate virus to test and evaluate new technologies and countermeasures. Herein we conducted a series of comparative evaluation in vitro studies between SVA and FMDV using the LF-BK α_Vβ₆ cell line. These include utilization of LF-BK α_Vβ₆ cells for field virus isolation, production of high virus titers, and evaluating serological reactivity and virus susceptibility to porcine type I interferons. These four methodologies utilizing LF-BK α_Vβ₆ cells were applicable to research with SVA and results support the current use of SVA as a surrogate for FMDV.

Keywords: FMDV; LF-BK αVβ6; Senecavirus A; cell line; interferon; isolation; swine.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cattle
Cell Line
Foot-and-Mouth Disease Virus*
Foot-and-Mouth Disease*
Integrins
Interferon Type I*
Picornaviridae*
Swine
Swine Diseases*

Substances

Integrins
Interferon Type I

Supplementary concepts

Senecavirus A

Grants and funding

This research was funded by the U.S. DHS Science and Technology Directorate (S&T), and SAIC was funded through an agreement under contract 70RSAT19D00000004.