New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum

Zeiad Moussa; Ehsan M Rashad; Elsherbiny A Elsherbiny; Abdulaziz A Al-Askar; Amr Abker Arishi; Fatimah O Al-Otibi; WesamEldin I A Saber

doi:10.3390/microorganisms10091814

New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum

Microorganisms. 2022 Sep 10;10(9):1814. doi: 10.3390/microorganisms10091814.

Authors

Zeiad Moussa¹, Ehsan M Rashad², Elsherbiny A Elsherbiny³, Abdulaziz A Al-Askar⁴, Amr Abker Arishi⁵, Fatimah O Al-Otibi⁴, WesamEldin I A Saber¹

Affiliations

¹ Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt.
² Department of Seed Pathology Research, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.
³ Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
⁴ Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁵ School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia.

Abstract

Ralstonia solanacearum is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of R. solanacearum can act as a biocontrol mediator for managing potato bacterial wilt. Virulent R. solanacearum was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers' treatments by the avirulent strain of R. solanacearum, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC-MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent R.solanacearum strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic.

Keywords: GC–MS analysis; bacterial supernatant; biological control; defense-related enzymes; lytic enzymes.

Grants and funding

This research project was supported by a grant from the Researchers Supporting Project number (RSP-2021/114), King Saud University, Riyadh, Saudi Arabia.