Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion

Chun-Wei Wu; Jia-Jin Chen; Chou-Ching K Lin; Chien-An Chen; Chun-Ie Wu; Ing-Shiou Hwang; Tsung-Hsun Hsieh; Bor-Shing Lin; Chih-Wei Peng

doi:10.3390/ijms231810318

Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion

Int J Mol Sci. 2022 Sep 7;23(18):10318. doi: 10.3390/ijms231810318.

Authors

Chun-Wei Wu¹, Jia-Jin Chen², Chou-Ching K Lin³, Chien-An Chen², Chun-Ie Wu², Ing-Shiou Hwang^{4

5}, Tsung-Hsun Hsieh^{6

7}, Bor-Shing Lin⁸, Chih-Wei Peng^{1

9}

Affiliations

¹ School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
² Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
³ Department of Neurology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
⁴ Institute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
⁵ Department of Physical Therapy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
⁶ Department of Physical Therapy and Graduate Institute of Rehabilitation Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
⁷ Neuroscience Research Center, Chang Gung Memorial Hospital, Linkou 33305, Taiwan.
⁸ Department of Computer Science and Information Engineering, National Taipei University, New Taipei City 237303, Taiwan.
⁹ School of Gerontology and Long-Term Care, College of Nursing, Taipei Medical University, Taipei 11031, Taiwan.

Abstract

Various infarct sizes induced by middle cerebral artery occlusion (MCAO) generate inconsistent outcomes for stroke preclinical study. Monitoring cerebral hemodynamics may help to verify the outcome of MCAO. The aim of this study was to investigate the changes in brain tissue optical properties by frequency-domain near-infrared spectroscopy (FD-NIRS), and establish the relationship between cerebral hemodynamics and infarct variation in MCAO model. The rats were undergone transient MCAO using intraluminal filament. The optical properties and hemodynamics were measured by placing the FD-NIRS probes on the scalp of the head before, during, and at various time-courses after MCAO. Bimodal infarction severities were observed after the same 90-min MCAO condition. Significant decreases in concentrations of oxygenated hemoglobin ([HbO]) and total hemoglobin ([HbT]), tissue oxygenation saturation (StO₂), absorption coefficient (μa) at 830 nm, and reduced scattering coefficient (μs') at both 690 and 830 nm were detected during the occlusion in the severe infarction but not the mild one. Of note, the significant increases in [HbO], [HbT], StO₂, and μa at both 690 and 830 nm were found on day 3; and increases in μs' at both 690 and 830 nm were found on day 2 and day 3 after MCAO, respectively. The interhemispheric correlation coefficient (IHCC) was computed from low-frequency hemodynamic oscillation of both hemispheres. Lower IHCCs standing for interhemispheric desynchronizations were found in both mild and severe infarction during occlusion, and only in severe infarction after reperfusion. Our finding supports that sequential FD-NIRS parameters may associated with the severity of the infarction in MCAO model, and the consequent pathologies such as vascular dysfunction and brain edema. Further study is required to validate the potential use of FD-NIRS as a monitor for MCAO verification.

Keywords: absorption; cerebral blood flow; cerebral hemodynamics; interhemispheric correlation coefficient; ischemic stroke; middle cerebral artery occlusion; near infrared spectroscopy; scattering.

MeSH terms

Animals
Disease Models, Animal
Hemodynamics
Infarction, Middle Cerebral Artery* / pathology
Oxyhemoglobins
Rats
Stroke* / pathology

Substances

Oxyhemoglobins

Abstract

MeSH terms

Substances

Grants and funding