Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

Yixin Wu; Mingzheng Wu; Abraham Vázquez-Guardado; Joohee Kim; Xin Zhang; Raudel Avila; Jin-Tae Kim; Yujun Deng; Yongjoon Yu; Sarah Melzer; Yun Bai; Hyoseo Yoon; Lingzi Meng; Yi Zhang; Hexia Guo; Liu Hong; Evangelos E Kanatzidis; Chad R Haney; Emily A Waters; Anthony R Banks; Ziying Hu; Ferrona Lie; Leonardo P Chamorro; Bernardo L Sabatini; Yonggang Huang; Yevgenia Kozorovitskiy; John A Rogers

doi:10.1038/s41467-022-32947-0

Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

Nat Commun. 2022 Sep 22;13(1):5571. doi: 10.1038/s41467-022-32947-0.

Authors

Yixin Wu^#^{1

2

3}, Mingzheng Wu^#⁴, Abraham Vázquez-Guardado^#^{2

3}, Joohee Kim^#^{2

3

5}, Xin Zhang⁴, Raudel Avila⁶, Jin-Tae Kim^{2

3}, Yujun Deng^{6

7}, Yongjoon Yu⁸, Sarah Melzer⁹, Yun Bai^{1

2

3}, Hyoseo Yoon⁴, Lingzi Meng^{1

2

3}, Yi Zhang^{10

11}, Hexia Guo^{1

2

3}, Liu Hong¹², Evangelos E Kanatzidis^{2

3

13}, Chad R Haney¹⁴, Emily A Waters¹⁴, Anthony R Banks^{2

8}, Ziying Hu^{2

3}, Ferrona Lie⁸, Leonardo P Chamorro¹², Bernardo L Sabatini⁹, Yonggang Huang^{15

16

17}, Yevgenia Kozorovitskiy^{18

19}, John A Rogers^{20

21

22

23

24

25

26

27

28

29}

Affiliations

¹ Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA.
² Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA.
³ Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA.
⁴ Department of Neurobiology, Northwestern University, Evanston, IL, USA.
⁵ Center for Bionics of Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
⁶ Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.
⁷ State Key Laboratory of Mechanical System and Vibration, Shanghai Jiao Tong University, Shanghai, China.
⁸ Neurolux Inc, Northfield, IL, USA.
⁹ Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, 220 Longwood Ave, Boston, MA, 02115, USA.
¹⁰ Polymer Program, Institute of Materials Science, University of Connecticut, Storrs, CT, 06269, US.
¹¹ Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, US.
¹² Mechanical Science and Engineering Department, University of Illinois, Urbana, IL, USA.
¹³ Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA.
¹⁴ Center for Advanced Molecular Imaging, Radiology, and Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
¹⁵ Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA. y-huang@northwestern.edu.
¹⁶ Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA. y-huang@northwestern.edu.
¹⁷ Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, USA. y-huang@northwestern.edu.
¹⁸ Department of Neurobiology, Northwestern University, Evanston, IL, USA. yevgenia.kozorovitskiy@northwestern.edu.
¹⁹ Chemistry of Life Processes Institutes, Northwestern University, Evanston, IL, USA. yevgenia.kozorovitskiy@northwestern.edu.
²⁰ Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²¹ Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²² Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²³ Neurolux Inc, Northfield, IL, USA. jrogers@northwestern.edu.
²⁴ Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁵ Simpson Querrey Institute & Feinberg Medical School, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁶ Department of Chemistry, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁷ Department of Neurological Surgery, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁸ Department of Electrical and Computer Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁹ Department of Computer Science, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.

^# Contributed equally.

Abstract

In vivo optogenetics and photopharmacology are two techniques for controlling neuronal activity that have immense potential in neuroscience research. Their applications in tether-free groups of animals have been limited in part due to tools availability. Here, we present a wireless, battery-free, programable multilateral optofluidic platform with user-selected modalities for optogenetics, pharmacology and photopharmacology. This system features mechanically compliant microfluidic and electronic interconnects, capabilities for dynamic control over the rates of drug delivery and real-time programmability, simultaneously for up to 256 separate devices in a single cage environment. Our behavioral experiments demonstrate control of motor behaviors in grouped mice through in vivo optogenetics with co-located gene delivery and controlled photolysis of caged glutamate. These optofluidic systems may expand the scope of wireless techniques to study neural processing in animal models.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / physiology
Glutamates
Mice
Neurosciences*
Optogenetics* / methods
Wireless Technology

Substances

Glutamates

Abstract

Publication types

MeSH terms

Substances

Grants and funding