Real-time kinetics and affinity analysis of the interaction between protein A and immunoglobulins G derived from different species on silica colloidal crystal films

Lu Wang; Yizhen Wan; Ning Ma; Lele Zhou; Dongmin Zhao; Jianning Yu; Huili Wang; Zhiping Lin; Weiping Qian

doi:10.1016/j.colsurfb.2022.112839

Real-time kinetics and affinity analysis of the interaction between protein A and immunoglobulins G derived from different species on silica colloidal crystal films

Colloids Surf B Biointerfaces. 2022 Nov:219:112839. doi: 10.1016/j.colsurfb.2022.112839. Epub 2022 Sep 13.

Authors

Lu Wang¹, Yizhen Wan¹, Ning Ma¹, Lele Zhou¹, Dongmin Zhao², Jianning Yu², Huili Wang², Zhiping Lin³, Weiping Qian⁴

Affiliations

¹ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
² Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
³ Nanjing Weigang Dairy Co., Ltd., Nanjing 211102, China.
⁴ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: wqian@seu.edu.cn.

PMID: 36137338
DOI: 10.1016/j.colsurfb.2022.112839

Abstract

Kinetic and affinity analysis of protein interactions reveals information on their related activities in biological processes. Herein, we established a system for evaluating the kinetics and affinity of the interaction between protein A and various IgG species on the surface of silica spheres of silica colloidal crystal (SCC) films by the extraordinary optical interference capabilities of 190 nm silica spheres after self-assembly. The equilibrium association constant (KA) was calculated by the equilibrium Langmuir model and nonlinear least-squares analysis of time-dependent data. The relative protein A/IgG binding affinity is human > rabbit >cow >goat. In addition, the competitive interaction of distinct species of IgG with protein A at the interface of SCC films was studied and performed. These findings may help with the use of protein A and other recognition components in a number of sensor types. Furthermore, this research might offer a novel approach to determining the kinetics and affinity of proteins on the surface of spheres particles, which may contribute to the development of the application of spheres particles in pharmaceutical science, biomedical engineering, and other techniques.

Keywords: Affinity; Immunoglobulins G; Kinetics; Protein A; Silica colloidal crystal films.