Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells

Zhenbin Bi; Xuezhu Gu; Yeyi Xiao; Yajing Zhou; Wenbin Bao; Shenglong Wu; Haifei Wang

doi:10.3390/toxins14090639

Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells

Toxins (Basel). 2022 Sep 16;14(9):639. doi: 10.3390/toxins14090639.

Authors

Zhenbin Bi¹, Xuezhu Gu², Yeyi Xiao¹, Yajing Zhou¹, Wenbin Bao^{1

3}, Shenglong Wu^{1

3}, Haifei Wang^{1

3}

Affiliations

¹ Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
² Lvliang Central Animal Husbandry and Veterinary Station, Huaian 211600, China.
³ Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China.

Abstract

Zearalenone (ZEN) is one of the mycotoxins that pose high risks for human and animal health, as well as food safety. However, the regulators involved in ZEN cellular toxicity remain largely unknown. Herein, we showed that cell viability of porcine intestinal epithelial cells (IPEC-J2) tended to decrease with increasing doses of ZEN by the cell counting kit-8 assay. Expression of the ISLR2 (immunoglobulin superfamily containing leucine-rich repeat 2) gene in IPEC-J2 cells was significantly downregulated upon ZEN exposure. Furthermore, we found the dose-effect of ZEN on ISLR2 expression. We then overexpressed the ISLR2 gene and observed that overexpression of ISLR2 obviously reduced the effects of ZEN on cell viability, apoptosis rate and oxidative stress level. In addition, ISLR2 overexpression significantly decreased the expression of TNF-α and IFN-α induced by ZEN. Our findings revealed the effects of ZEN on the ISLR2 gene expression and indicated the ISLR2 gene as a novel regulator of ZEN-induced cytotoxicity, which provides potential molecular targets against ZEN toxicity.

Keywords: ISLR2 gene; cytotoxicity; molecular target; pig; zearalenone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Epithelial Cells
Humans
Immunoglobulins
Leucine
Mycotoxins* / toxicity
Swine
Tumor Necrosis Factor-alpha / genetics
Zearalenone* / toxicity

Substances

Immunoglobulins
Mycotoxins
Tumor Necrosis Factor-alpha
Zearalenone
Leucine

Grants and funding

This study was financially supported by Seed Industry Vitalization Research Projects of Jiangsu Province (JBGS [2021]024), Key Research and Development Project (Modern Agriculture) of Jiangsu Province (BE2019341), Qing Lan Project of Yangzhou University, and Priority Academic Program Development of Jiangsu Higher Education Institution.