Purification of quantum dot-based bioprobes with a salting out strategy

Zhi-Liang Chen; Jia-Quan Xu

doi:10.1039/d1na00569c

Purification of quantum dot-based bioprobes with a salting out strategy

Nanoscale Adv. 2022 Jan 4;4(2):393-396. doi: 10.1039/d1na00569c. eCollection 2022 Jan 18.

Authors

Zhi-Liang Chen¹, Jia-Quan Xu²

Affiliations

¹ School of Pharmacy, Shaoyang University Shaoyang 422000 P. R. China.
² Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology Nanchang 330013 China jiaquan_xu@foxmail.com +86-739-5308282.

Abstract

A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD.