SOX2 inhibits LLGL2 polarity protein in esophageal squamous cell carcinoma via miRNA-142-3p

Shihui Zhang; Yunyun Chen; Qiong Hu; Tingting Zhao; Zhuo Wang; Yijian Zhou; Yuxuan Wei; Hongzhou Zhao; Junkai Wang; Yaxin Yang; Jiaying Zhang; Songlin Shi; Yujun Zhang; Ling Yang; Zhichao Fu; Kuancan Liu

doi:10.1080/15384047.2022.2126248

SOX2 inhibits LLGL2 polarity protein in esophageal squamous cell carcinoma via miRNA-142-3p

Cancer Biol Ther. 2022 Dec 31;23(1):1-15. doi: 10.1080/15384047.2022.2126248.

Authors

Shihui Zhang^{1

2}, Yunyun Chen^{1

3}, Qiong Hu^{3

4}, Tingting Zhao^{1

3}, Zhuo Wang^{1

3}, Yijian Zhou^{1

3}, Yuxuan Wei^{1

3}, Hongzhou Zhao^{1

3}, Junkai Wang⁵, Yaxin Yang⁶, Jiaying Zhang⁵, Songlin Shi³, Yujun Zhang³, Ling Yang³, Zhichao Fu⁷, Kuancan Liu^{1

3}

Affiliations

¹ Central Laboratory, Xiang'an Hospital of Xiamen University, Xiamen, China.
² Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
³ School of Medicine, Xiamen University, Xiamen, China.
⁴ Department of Clinic Medical Laboratory, Zhoushan Hospital, Zhoushan, China.
⁵ School of Life Sciences, Xiamen University, Xiamen, China.
⁶ Department of Biology, University of Rochester, Rochester, New York, USA.
⁷ Department of radiotherapy, 900 Hospital of the Joint Logistics Team (Dongfang Hospital, Xiamen University), Fuzhou, China.

Abstract

CCK-8, Cell Counting Kit 8; Chip, Chromatin Immunoprecipitation; EC, Esophageal cancer; EMT, epithelial-to-mesenchymal transition; ESCC, Esophageal squamous cell carcinomas; LLGL2, lethal (2) giant larvae protein homolog 2; LLGL2ov, LLGL2 overexpression; MET, mesenchymal-epithelial transition; miRNAs, MicroRNAs; PRM-MS, Parallel reaction monitoring-Mass spectrometry; SD, Standard deviation; SOX, sex determining region Y (SRY)-like box; SOX2-Kd, SOX2-knockdwon; TUNEL, TdT-mediated dUTP Nick-End Labeling.

Keywords: Esophageal squamous cell carcinoma; LLGL2 protein; SOX2; cellular polarity; miR-142-3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Squamous Cell* / pathology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Esophageal Neoplasms* / pathology
Esophageal Squamous Cell Carcinoma* / genetics
Esophageal Squamous Cell Carcinoma* / pathology
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism

Substances

MicroRNAs
SOX2 protein, human
SOXB1 Transcription Factors

Grants and funding

The study was supported by the following grants: National Natural Science Foundation of China (No. 81772994 and 82273044 to K. L), the Natural Science Foundation of Fujian Province (No. 2021D034 and 2017I0014 to K. L), the Program from Xiang’an Hospital (No. PM202012220001 to K. L), the National High Technology Research and Development Program of China (No. 2014AA020541 to K. L), and the Program for the Top Young Innovative Talents of Fujian Province (No. 2016RCLKC to K. L).