Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations

Dong Fu

doi:10.1007/978-1-0716-2557-6_11

Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations

Methods Mol Biol. 2022:2544:159-169. doi: 10.1007/978-1-0716-2557-6_11.

Author

Dong Fu^{1

2}

Affiliations

¹ Division of Pharmacotherapy and Experimental Therapeutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dong.fu@fronteratherapeutics.com.
² In vivo Pharmacology and Toxicology, Frontera Therapeutics, Bedford, MA, USA. dong.fu@fronteratherapeutics.com.

PMID: 36125717
DOI: 10.1007/978-1-0716-2557-6_11

Abstract

Collagen sandwich culture of hepatocytes retains many in vivo-like properties and is used for many investigations in liver cell biology, and hepatic pharmacology and toxicology. This chapter describes the method of establishing collagen sandwich culture of hepatocytes in a glass bottom dish for image-based studies.

Keywords: Collagen sandwich culture; Confocal microscope; Glass bottom dish; Hepatocytes; Hepatotoxicity; Polarization; Polarized trafficking.

MeSH terms

Cell Culture Techniques* / methods
Cells, Cultured
Collagen
Hepatocytes*
Liver

Substances

Collagen