Citric acid secretion from rice roots contributes to reduction and immobilization of Cr(VI) by driving microbial sulfur and iron cycle in paddy soil

Abstract

Root exudates released by plants can promote microbial growth and activity, thereby affecting the transformation and availability of soil pollutants. However, the effects of the root exudates of rice plants on chromium (Cr) transformation in paddy soils and the underlying mechanisms are yet to be elucidated properly. The present study investigated how rice root exudates interact with rhizosphere microorganisms to influence the transformation of Cr and explored the key components in root exudates that affect Cr(VI) reduction. The results showed that the addition of root exudate and citric acid markedly decreased soil pH and increased dissolved organic carbon content that created favorable conditions and provided sufficient electron donors for Cr(VI) reduction, thereby greatly facilitating the reduction of Cr(VI) and the transformation of HOAc-extractable Cr into more stable oxidizable and residual Cr. Additionally, Desulfovibrio-related sulfate-reducing bacteria, Thiobacillus-related sulfide-oxidizing bacteria, and Geobacter-related Fe(III)-reducing bacteria were enriched with the addition of root exudate and citric acid. Among them, sulfate would be reduced by Desulfovibrio to sulfide, which would be further utilized by Thiobacillus to reduce Cr(VI), thereby enabling the continuous reduction of Cr(VI); simultaneously, Geobacter would sustain the reduction of Cr(VI) by reducing Fe(III) to Fe(II). Furthermore, based on the high-level secretion of citric acid in response to Cr(VI) exposure and the similar effects of root exudates and citric acid on Cr(VI) reduction, it is proposed that citric acid is the key component in rice root exudates that affects Cr(VI) reduction. These results suggest that root exudates (citric acid as the key component) contribute to the reduction and immobilization of Cr(VI) by driving microbial S and Fe cycles, with Desulfovibrio, Thiobacillus, and Geobacter being the keystone genera. The study provides a novel insight into the Fe/S/Cr co-transformation processes with microbial involvement, and the artificial root exudate mixtures designed to reduce Cr(VI).

Keywords: Citric acid; Cr(VI) reduction; Fe(III)-reducing bacteria; Root exudate; Sulfate-reducing bacteria; Sulfide-oxidizing bacteria.