Alhagi maurorum aqueous extract protects against norfloxacin-induced hepato-nephrotoxicity in rats

Hesham Ahmed Khalifa; Shimaa Ibrahim Shalaby; Ahmed Shaban Abdelaziz

doi:10.1016/j.chmed.2019.09.007

Alhagi maurorum aqueous extract protects against norfloxacin-induced hepato-nephrotoxicity in rats

Chin Herb Med. 2019 Dec 28;12(2):156-162. doi: 10.1016/j.chmed.2019.09.007. eCollection 2020 Apr.

Authors

Hesham Ahmed Khalifa¹, Shimaa Ibrahim Shalaby², Ahmed Shaban Abdelaziz¹

Affiliations

¹ Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
² Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.

Abstract

Objectives: While the protective effects of Alhagi maurorum have been shown against various ailments, its role against norfloxacin-induced adverse effects has not been studied. The current study was conducted to determine the effect of A. maurorum aqueous extract against norfloxacin-induced side effects in rats.

Methods: Twenty-four male albino rats were randomly assigned into four groups, which received normal saline, norfloxacin (50 mg/kg b.wt orally once a day), A. maurorum aqueous extract (300 mg/kg b.wt orally once a day), and norfloxacin with A. maurorum aqueous extract by the same previous mentioned dosages. Blood samples were collected for hematological examination to evaluate liver and kidney function tests. Hepatic and renal tissue samples were obtained to assess antioxidant activity and histopathological examination.

Results: A. maurorum aqueous extract significantly ameliorated norfloxacin-induced elevation in tissue malondialdehyde, and reduction in tissue antioxidant enzymes such as catalase, glutathione peroxidase, and superoxide dismutase activities as well as reduced glutathione concentration. Concurrent administration of A. maurorum aqueous extract with norfloxacin significantly reduced serum alkaline phosphatase, aminotransferases, urea, creatinine, and uric acid and increased RBCs count, Hb concentration, PCV, leucocyte, and monocyte counts compared with the norfloxacin-treated group. Co-administration of A. maurorum aqueous extract with norfloxacin prevented the degenerative changes induced by norfloxacin alone in liver and kidney tissues. The phytochemical profile of the extract showed the presence of carbohydrates, alkaloids, saponins, tannins, phenolics, and flavonoids.

Conclusion: These findings indicated that A. maurorum possesses potent antioxidant activities and could be used to attenuate norfloxacin-induced side effects.

Keywords: Alhagi maurorum Boiss; anti-oxidant enzymes; creatinine; malondialdehyde; serum alkaline phosphatase.