Antioxidation and active constituents analysis of flower residue of Rosa damascena

Wen-Ya Liu; Lin-Yu Chen; Ying-Ying Huang; Ling Fu; Le-Yuan Song; Yun-Yu Wang; Zhe Bai; Fang-Feng Meng; Yue-Feng Bi

doi:10.1016/j.chmed.2020.05.005

Antioxidation and active constituents analysis of flower residue of Rosa damascena

Chin Herb Med. 2020 Jul 11;12(3):336-341. doi: 10.1016/j.chmed.2020.05.005. eCollection 2020 Jul.

Authors

Wen-Ya Liu¹, Lin-Yu Chen¹, Ying-Ying Huang¹, Ling Fu^{1

2}, Le-Yuan Song¹, Yun-Yu Wang¹, Zhe Bai¹, Fang-Feng Meng¹, Yue-Feng Bi^{1

2}

Affiliations

¹ School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China.
² Collaborative Innovation Center of New Drug Research and Safety Evaluation, Zhengzhou 450001, China.

Abstract

Objective: To make full usage of resource and turn waste into treasure, the chemical constituents and bioactivity were firstly investigated on Damask rose (Rosa damascena) flower residue (DRFR).

Methods: DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR. Then, column chromatography was used to purify compounds from an antioxidation extract (DRFR-A), and the chemical structure was identified using NMR. The total phenolic acid content was measured by Folin-Ciocalteu colorimetric method, and the content of gallic acid of the indicator ingredient was detected by HPLC.

Results: DRFR-A was found to show a high activity both on DPPH (IC₅₀: 2.760 µg/mL) and ABTS (IC₅₀: 2.258 µg/mL) compared to positive control V_C. Ten compounds were isolated and identified as quercetin (1), kaempferol (2), gallic acid (3), protocatechuic acid (4), pyrogallic acid (5), 2-phenylethyl 3,4,5-trihydroxybenzoate (6), methyl gallate (7), p-hydroxybenzoic acid (8), p-hydroxyphenethyl alcohol (9) and astragalin (10) from DRFR-A. Among them, pyrogallic acid, 2-phenylethyl-3, 4, 5-trihydroxybenzoate, p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time. The content of total phenolic acids and gallic acid, main ingredient in DRFR-A was determined as 63.73% and 24.67%, respectively.

Conclusion: This study provides a reliable data and lays the foundation for the development and utilization of rose residue, and hence for the full utilization of rose resources.

Keywords: Damask rose flower residue; HPLC; Rosa damascena Mill; antioxidant activity; phenolic acids; phenolics.