Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens

Eman M Sharaf; Amr Hassan; Fawziah A Al-Salmi; Fauzeya M Albalwe; Hessa Meteq R Albalawi; Doaa B Darwish; Eman Fayad

doi:10.3389/fmicb.2022.929491

Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens

Front Microbiol. 2022 Sep 2:13:929491. doi: 10.3389/fmicb.2022.929491. eCollection 2022.

Authors

Eman M Sharaf¹, Amr Hassan², Fawziah A Al-Salmi³, Fauzeya M Albalwe⁴, Hessa Meteq R Albalawi⁵, Doaa B Darwish⁶, Eman Fayad⁷

Affiliations

¹ Department of Bacteriology, Immunology, and Mycology, Animal Health Research Institute (AHRI), Shebin El Kom, Egypt.
² Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat, Egypt.
³ Department of Biology, Faculty of Sciences, Taif University, Taif, Saudi Arabia.
⁴ Department of Biology, Faculty of Science, Tabuk University, Tabuk, Saudi Arabia.
⁵ Department of Pharmacy, Prince Sultan Armed Forces Hospital, Medina, Saudi Arabia.
⁶ Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt.
⁷ Department of Biotechnology, Faculty of Sciences, Taif University, Taif, Saudi Arabia.

Abstract

The development of innovative antibacterial drugs against foodborne pathogens has led to an interest in novel materials such as nanomaterials. The unique features of nanomaterial qualify it for use as an antibacterial treatment. Noble metals and metal oxide nanoparticles, such as silver and magnetite nanoparticles, have been shown to be effective antibacterial medications against a range of microorganisms. In this work, Ag@Fe₃O₄ -NPs were fabricated by using a wet chemical reduction and modified co-precipitation techniques. The antibacterial efficiency of the Ag/Fe₃O₄ core shell nanoparticles was investigated by applying various techniques, such as the Kirby-Bauer Disk Diffusion test, minimum inhibitory concentration (MIC) and bactericidal concentration (MBC), Colony Forming Unit (CFU), and kill time assay. The toxicity mechanism of Ag@Fe₃O₄ -NPs against Salmonella typhimurium and Escherichia coli was studied by apoptosis and reactive oxygen species (ROS) assays. The data revealed that a cubic core was surrounded by a silver shell, which indicated the regular morphology of silver magnetite core shell nanoparticles without any aggregation. Furthermore, Ag@Fe₃O₄ -NPs is more toxic against S. typhimurium and E. coli than Ag-NPs and Fe₃O₄ NPs. The MIC values for Ag/Fe₃O₄ NPs against S. typhimurium and E. coli were 3.1 and 5.4 μg/ml, respectively, whereas the MIC values for Ag-NPs and MNPs against S. typhimurium and E. coli were 4.1 and 8.2 μg/ml for Ag-NPs and 6.9 and 10.3 μg/ml for MNPs. The results showed the ability of Ag@Fe₃O₄ -NPs to induce apoptosis by generating ROS. Also, the ability of Ag@Fe₃O₄ -NPs to liberate free Ag⁺ and generate ROS via the Haber-Weiss cycle may be a plausible mechanism to explain the toxicity of Ag@Fe₃O₄ -NPs - NPs.

Keywords: Escherichia coli; Gram negative; Salmonella typhimurium; apoptosis; foodborne pathogens; reactive oxygen species; silver/magnetite core shell nanoparticle.