Objective: To characterize the species of invasive Pomacea snails that were discovered for the first time in Shandong Province.
Methods: Pomacea snails samples were collected in the field of Jining City, Shandong Province on October 2021 for morphological identification. Pomacea snails were randomly sampled and genomic DNA was extracted from foot muscle tissues of Pomacea snails for multiplex PCR amplification. The PCR amplification product was sequenced. Then, the sequence was aligned and a phylogenetic tree was created using the software MegAlign 7.1.0. In addition, Angiostongylus cantonensis infection was detected in Pomacea snails with the lung microscopy.
Results: A total of 104 living Pomacea snails were collected, and all were characterized as Pomacea spp. based on morphological features. Of 12 randomly selected adult Pomacea snails, multiplex PCR assay and sequencing identified eleven snails as P. canaliculata and one as P. maculata. No A. cantonensis infection was detected in 104 Pomacea snails.
Conclusions: This is the first report of invasive Pomacea snails in Shandong Province, where P. canaliculata and P. maculata are found.
［摘要］ 目的 对山东省境内首次发现的入侵生物福寿螺进行物种鉴定。方法 2021 年 10 月, 在山东省济宁市野外采 集福寿螺样本进行形态学鉴定; 随机选取部分福寿螺样本, 取螺腹足部肌肉组织提取基因组 DNA 进行多重 PCR 扩增并 测序, 利用 MegAlign 7.1.0 软件对测序后的序列进行比对并构建系统发育树。采用肺检法检测福寿螺中广州管圆线虫感 染情况。结果 共采集福寿螺活体样本 104 只, 经形态学鉴定均为福寿螺属物种; 随机挑选 12 只成螺样本, 经多重 PCR和测序鉴定 11 只为小管福寿螺、1 只为斑点福寿螺。104 只福寿螺均未发现广州管圆线虫感染。结论 山东省境内首次 发现福寿螺入侵, 且存在小管福寿螺和斑点福寿螺。.
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