Previous studies on stationary phase gradients have inspired a new phase of theoretical work and an expansion of the concept to include multiple retention mechanisms. The theoretical work presented here corroborates previous reports that a stationary phase gradient can produce selectivity in a separation as effectively as careful adjustments to a mobile phase gradient program, especially when such column is operated in mobile phase gradient elution mode. In reality, no column is singularly based on one type of interaction mechanism, and many columns are nowadays designed to solicit multiple solute to stationary phase interactions. These mixed-mode columns inspired us to give more careful consideration to the idea of dual stationary phase gradients. With the theory applied here, it is suggested that the modulation of two unique interaction mechanisms across a chromatographic column has the potential to open up previously unseen selectivities. With the increasing prevalence of mixed-mode columns, we believe there will be ample opportunity to explore these new concepts in experimental work.
Keywords: Dual gradient; Large molecules; Mixed-mode; Selectivity modeling; Stationary phase gradient.
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