Fungus-algae symbiotic systems (FASS) are typically used to assist in the immobilization of algae and strengthen the adsorption of heavy metals. However, the adsorption behavior of the symbiotic system and the molecular regulation mechanism of extracellular proteins in the adsorption of heavy metals have not been reported in detail. In this study, a stable FCSS (fungus-cyanobacterium symbiotic system) was used to study Cd(II) adsorption behavior. The fixation efficiency of fungus to cyanobacterium reached more than 95% at pH7.0, 30 °C, 150 rpm, and a medium ratio of 100%. The biomass, chlorophyll content, and total fatty acid content of the symbiotic system were much higher than those of cyanobacterium and fungus alone. The photosynthetic fluorescence parameters showed that the presence of fungus enhanced the light tolerance of cyanobacterium. The original light energy conversion efficiency and potential activity of PSII were enhanced, indicating that symbiosis could promote the photosynthetic process of cyanobacterium. The Cd(II) adsorption efficiency can achieve 90%. The system maintained excellent adsorption after six adsorption cycles. Differential proteins were mainly enriched in areas such as metabolism, ABC transport system, and pressure response. Cd(II) stress promotes an increase in efflux proteins. Moreover, cadmium can be fixed as much as possible by secreting extracellular proteins, and the toxicity of cadmium to cells can be alleviated by regulating the metabolism of glutathione, reducing oxidative phosphorylation level, and reducing oxidative stress, thus improving the resistance to Cd(II). Meanwhile, the expression of enzymes involved in glycolysis and the pentose phosphate pathway was upregulated, while the expression of those in the TCA cycle was downregulated. The expression of substances related to PSI and PSII in the photosynthetic system and rubisco, a key enzyme in the Calvin cycle, was significantly upregulated, indicating that the glucose metabolism and photosynthetic pathways of the symbiotic system were involved in resistance to Cd toxicity. This revealed the response mechanism of the fungus-algal symbiotic system in the process of Cd adsorption, and also provided reference value for industrial application in water treatment.
Keywords: Adsorption; Cadmium; Extracellular protein; Proteomics; Symbiotic system; Synechocystis sp. PCC6803.
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